biostudies-literatureCalvo-Lozano OConsiglio Nazionale delle RicercheGeneralitat de CatalunyaEuropean Social FundCompetitivity Operational ProgramMinisterio de Ciencia e Innovaci?nAgencia Estatal de Investigaci?n14659-14665https://www.ebi.ac.uk/biostudies/studies/S-EPMC9607850biostudies-literatureUnknown94(42)The rapid diagnosis of cancer, especially in its early stages, is crucial for on-time medical treatment and for increasing the patient survival rate. Lung cancer shows the highest mortality rate and the lowest 5-year survival rate due to the late diagnosis in advanced cancer stages. Providing rapid and reliable diagnostic tools is a top priority to address the problem of a delayed cancer diagnosis. We introduce a nanophotonic biosensor for the direct and real-time detection in human plasma of the microRNA-21-5p biomarker related to lung cancer. The biosensor employs a silicon photonic bimodal interferometric waveguide that provides a highly sensitive detection in a label-free format. We demonstrate a very competitive detectability for direct microRNA-21-5p biomarker assays in human plasma Analytical chemistryOne-Step and Real-Time Detection of microRNA-21 in Human Samples for Lung Cancer Biosensing Diagnosis.PMC9607850SEV-2017-0706POC-P_37_796/2016PCI2019-111890-2PID2019-105132RB-I00BES-2017-080527TEC2016-78515-REstevez MCCalvo-Lozano OGarcia-Aparicio PBerindan-Neagoe IFerracin MLechuga LMRaduly LZfalseOne-Step and Real-Time Detection of microRNA-21 in Human Samples for Lung Cancer Biosensing Diagnosis.The rapid diagnosis of cancer, especially in its early stages, is crucial for on-time medical treatment and for increasing the patient survival rate. Lung cancer shows the highest mortality rate and the lowest 5-year survival rate due to the late diagnosis in advanced cancer stages. Providing rapid and reliable diagnostic tools is a top priority to address the problem of a delayed cancer diagnosis. We introduce a nanophotonic biosensor for the direct and real-time detection in human plasma of the microRNA-21-5p biomarker related to lung cancer. The biosensor employs a silicon photonic bimodal interferometric waveguide that provides a highly sensitive detection in a label-free format. We demonstrate a very competitive detectability for direct microRNA-21-5p biomarker assays in human plasma 2022-01-01T00:00:00Z2022 Oct2025-05-18T11:38:36.313Z2025-05-18T11:38:36.313ZS-EPMC96078503621956510.1021/acs.analchem.2c02895