biostudies-literatureSantos ALSão Paulo Research FoundationFundação de Amparo à Pesquisa do Estado de São Paulo—FAPESPFundação de Amparo à Pesquisa do Estado de Minas Gerais FAPEMIG903https://www.ebi.ac.uk/biostudies/studies/S-EPMC9611219biostudies-literatureUnknown12(10)Endophytes have been shown to be a source of novel drug prototypes. The <i>Casearia</i> genus is known for presenting cytotoxic clerodane diterpenes; however, there are few reports on secondary metabolites produced by its fungal microbiota. Thus, in the present study endophytic fungi obtained from the fresh leaves of <i>C. arborea</i> were grown in potato dextrose broth and rice to perform a secondary metabolite prospection study. The cytotoxic profile of the crude extracts at 10 µg/mL was determined by a colorimetric assay on tumor cell lines. The endophytes producing cytotoxic extracts were identified through phylogenetic analysis and belong to <i>Diaporthe</i> and <i>Colletotrichum</i> species. Metabolites present in these extracts were organized in molecular networking format based on MetabolitesDereplication of Cytochalasans and Octaketides in Cytotoxic Extracts of Endophytic Fungi from <i>Casearia arborea</i> (Salicaceae).PMC96112192016/24985-42016/24985-4,APQ 02036-21APQ 00207-182020/16554-9Santos ALHorvath ROSoares MGIonta MFerreira MJPSartorelli PSilva DOKawafune ESfalseDereplication of Cytochalasans and Octaketides in Cytotoxic Extracts of Endophytic Fungi from <i>Casearia arborea</i> (Salicaceae).Endophytes have been shown to be a source of novel drug prototypes. The <i>Casearia</i> genus is known for presenting cytotoxic clerodane diterpenes; however, there are few reports on secondary metabolites produced by its fungal microbiota. Thus, in the present study endophytic fungi obtained from the fresh leaves of <i>C. arborea</i> were grown in potato dextrose broth and rice to perform a secondary metabolite prospection study. The cytotoxic profile of the crude extracts at 10 µg/mL was determined by a colorimetric assay on tumor cell lines. The endophytes producing cytotoxic extracts were identified through phylogenetic analysis and belong to <i>Diaporthe</i> and <i>Colletotrichum</i> species. Metabolites present in these extracts were organized in molecular networking format based on 2022-01-01T00:00:00Z2022 Sep2026-04-08T18:22:09.074Z2025-04-19T04:24:29.34ZS-EPMC96112193629580510.3390/metabo12100903