biostudies-literatureWang SMajor International S&amp;T Cooperation Project, Ministry of Science and Technology of the People's Republic of ChinaKey Project of NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine896https://www.ebi.ac.uk/biostudies/studies/S-EPMC9612088biostudies-literatureUnknown12(10)<i>Glycyrrhiza glabra</i> L., known as licorice, is one of the most famous herbs in the world. In this study, we investigated the phytochemical and antitumor activities of <i>G. glabra</i>, especially its anti-colorectal cancer activities. <i>G. glabra</i> was extracted with 70% methanol, and the ethyl acetate layer was separated by silica gel, ODS, LH-20 column chromatography, and semi-preparative HPLC to obtain the compounds. The structures were determined by NMR and MS methods. Three new compounds named licopyranol A-C (<b>1</b>-<b>3</b>), and eighteen known compounds (<b>4</b>-<b>21</b>) were isolated. Compounds with an isoprenyl group or dimethylpyran ring showed better antitumor activities. Licopyranol A (<b>1</b>) and glycyrol (<b>5</b>) both inhibited the proliferation, reduced cloMetabolitesThree New Compounds, Licopyranol A-C, Together with Eighteen Known Compounds Isolated from <i>Glycyrrhiza glabra</i> L. and Their Antitumor Activities.PMC96120882020GSMPA-KL112016YFE0129000Jiang YLiu YChen QLi YYang ZHe DBi CWang SShen XAlafifi JHWu YfalseThree New Compounds, Licopyranol A-C, Together with Eighteen Known Compounds Isolated from <i>Glycyrrhiza glabra</i> L. and Their Antitumor Activities.<i>Glycyrrhiza glabra</i> L., known as licorice, is one of the most famous herbs in the world. In this study, we investigated the phytochemical and antitumor activities of <i>G. glabra</i>, especially its anti-colorectal cancer activities. <i>G. glabra</i> was extracted with 70% methanol, and the ethyl acetate layer was separated by silica gel, ODS, LH-20 column chromatography, and semi-preparative HPLC to obtain the compounds. The structures were determined by NMR and MS methods. Three new compounds named licopyranol A-C (<b>1</b>-<b>3</b>), and eighteen known compounds (<b>4</b>-<b>21</b>) were isolated. Compounds with an isoprenyl group or dimethylpyran ring showed better antitumor activities. Licopyranol A (<b>1</b>) and glycyrol (<b>5</b>) both inhibited the proliferation, reduced clo2022-01-01T00:00:00Z2022 Sep2025-05-29T14:37:18.741Z2024-10-19T01:42:43.448ZS-EPMC96120883629579810.3390/metabo12100896