{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gonzalez-Gomez L"],"funding":["MCIU/AEI/FEDER, UE","FCT-Fundação para a Ciência e a Tecnologia","ARDITI-Agência Regional para o Desenvolvimento da Investigação Tecnologia e Inovação","Fundação para a Ciência e Tecnologia and Madeira 14-2020 program to the Portuguese Mass Spectrometry Network through PROEQUIPRAM program"],"pagination":["650"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9612249"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(10)"],"pubmed_abstract":["This work presents an optimized methodology based on the miniaturization of the original QuEChERS (μ-QuEChERS) followed by liquid chromatography coupled to mass spectrometry (HPLC-MS/MS) for the determination of tropane alkaloids (TAs), atropine, and scopolamine in leafy vegetable samples. The analytical methodology was successfully validated, demonstrating quantitation limits (MQL) ≤ 2.3 ng/g, good accuracy, and precision, with recoveries between 90-100% and RSD ≤ 13% for both analytes. The method was applied to the analysis of TA-producing plants (<i>Brugmansia versicolor</i>, <i>Solandra maxima,</i> and <i>Convolvulus arvensis</i>). High concentrations of scopolamine were found in flowers (1771 mg/kg) and leaves (297 mg/kg) of <i>B. versicolor</i>. The highest concentration of atropine "],"journal":["Toxins"],"pubmed_title":["Improved Analytical Approach for Determination of Tropane Alkaloids in Leafy Vegetables Based on µ-QuEChERS Combined with HPLC-MS/MS."],"pmcid":["PMC9612249"],"funding_grant_id":["CQM Base Fund - UIDB/00674/2020 and Programmatic Fund - UIDP/00674/2020","M14-20 M1420-01-0145-FEDER-000008","RTI2018-094558-B-I00 (EVALKALIM)","M1420-01-0145-FEDER-000005 - Centro de Química da Madeira - CQM+ (Madeira 14-20 Program) and by the project M1420-09-5369-FSE-000001"],"pubmed_authors":["Gonzalez-Gomez L","Camara JS","Pereira JAM","Morante-Zarcero S","Sierra I"],"additional_accession":[]},"is_claimable":false,"name":"Improved Analytical Approach for Determination of Tropane Alkaloids in Leafy Vegetables Based on µ-QuEChERS Combined with HPLC-MS/MS.","description":"This work presents an optimized methodology based on the miniaturization of the original QuEChERS (μ-QuEChERS) followed by liquid chromatography coupled to mass spectrometry (HPLC-MS/MS) for the determination of tropane alkaloids (TAs), atropine, and scopolamine in leafy vegetable samples. The analytical methodology was successfully validated, demonstrating quantitation limits (MQL) ≤ 2.3 ng/g, good accuracy, and precision, with recoveries between 90-100% and RSD ≤ 13% for both analytes. The method was applied to the analysis of TA-producing plants (<i>Brugmansia versicolor</i>, <i>Solandra maxima,</i> and <i>Convolvulus arvensis</i>). High concentrations of scopolamine were found in flowers (1771 mg/kg) and leaves (297 mg/kg) of <i>B. versicolor</i>. The highest concentration of atropine ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2025-04-18T21:22:14.036Z","creation":"2025-04-07T09:16:01.757Z"},"accession":"S-EPMC9612249","cross_references":{"pubmed":["36287919"],"doi":["10.3390/toxins14100650"]}}