{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Martinez-Miguel M"],"funding":["Centro de Investigaci?n Biom?dica en Red en Bioingenier?a, Biomateriales y Nanomedicina","H2020 Marie Sklodowska-Curie Actions","European Social Fund","Ministerio de Ciencia, Innovaci?n y Universidades","H2020 Leadership in Enabling and Industrial Technologies","European Cooperation in Science and Technology","Universitat Aut?noma de Barcelona","Generalitat de Catalunya","Instituto de Salud Carlos III","Fundaci? la Marat? de TV3","Ag?ncia de Gesti? d?Ajuts Universitaris i de Recerca","Max-Planck-Gesellschaft","Ministerio de Econom?a y Competitividad","European Regional Development Fund"],"pagination":["48179-48193"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9614722"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(42)"],"pubmed_abstract":["The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle's fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy."],"journal":["ACS applied materials & interfaces"],"pubmed_title":["Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion."],"pmcid":["PMC9614722"],"funding_grant_id":["PID2020-115296RA-I00","CEX2019-000917-S","CA15126","CB/06/0074","SAF2014-60138-R","PDC2021-121481-I00","RYC-2017-22614","PID2019-105622RBI00","BBN20PIV02","201812","CTQ2015-66194-R","2017-SGR-1442","2017-SGR-229","RTI2018-093831-B-I00","801342","720942","2020FI_B2 00137","2017-SGR-1439","MAT2016-80826-R","2017-SGR-918","PID2019-111682RB-I00","101007804","953110","BBN18PI01"],"pubmed_authors":["Vargas-Nadal G","Royo M","Giannotti MI","Cristobal-Lecina E","Pulido D","Martinez-Miguel M","Kober M","Mas-Torrent M","Tomsen-Melero J","Ratera I","Castellote-Borrell M","Munoz J","Ventosa N","Kyvik AR","Guasch J","Veciana J","Passemard S"],"additional_accession":[]},"is_claimable":false,"name":"Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion.","description":"The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle's fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-22T02:01:41.26Z","creation":"2025-04-05T20:14:42.416Z"},"accession":"S-EPMC9614722","cross_references":{"pubmed":["36251059"],"doi":["10.1021/acsami.2c10497"]}}