biostudies-literatureMartinez-Miguel MCentro de Investigaci?n Biom?dica en Red en Bioingenier?a, Biomateriales y NanomedicinaH2020 Marie Sklodowska-Curie ActionsEuropean Social FundMinisterio de Ciencia, Innovaci?n y UniversidadesH2020 Leadership in Enabling and Industrial TechnologiesEuropean Cooperation in Science and TechnologyUniversitat Aut?noma de BarcelonaGeneralitat de CatalunyaInstituto de Salud Carlos IIIFundaci? la Marat? de TV3Ag?ncia de Gesti? d?Ajuts Universitaris i de RecercaMax-Planck-GesellschaftMinisterio de Econom?a y CompetitividadEuropean Regional Development Fund48179-48193https://www.ebi.ac.uk/biostudies/studies/S-EPMC9614722biostudies-literatureUnknown14(42)The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle's fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy.ACS applied materials & interfacesHierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion.PMC9614722PID2020-115296RA-I00CEX2019-000917-SCA15126CB/06/0074SAF2014-60138-RPDC2021-121481-I00RYC-2017-22614PID2019-105622RBI00BBN20PIV02201812CTQ2015-66194-R2017-SGR-14422017-SGR-229RTI2018-093831-B-I008013427209422020FI_B2 001372017-SGR-1439MAT2016-80826-R2017-SGR-918PID2019-111682RB-I00101007804953110BBN18PI01Vargas-Nadal GRoyo MGiannotti MICristobal-Lecina EPulido DMartinez-Miguel MKober MMas-Torrent MTomsen-Melero JRatera ICastellote-Borrell MMunoz JVentosa NKyvik ARGuasch JVeciana JPassemard SfalseHierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion.The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle's fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy.2022-01-01T00:00:00Z2022 Oct2025-04-22T02:01:41.26Z2025-04-05T20:14:42.416ZS-EPMC96147223625105910.1021/acsami.2c10497