biostudies-literatureHill JCNHLBI NIH HHSNational Institutes of Healthezac237https://www.ebi.ac.uk/biostudies/studies/S-EPMC9615433biostudies-literatureUnknown62(5)<h4>Objectives</h4>We hypothesized that expression and activity of nitric oxide synthase-3 enzyme (Nos3) in bicuspid aortic valve (BAV) aortopathy are related to tissue layer and Nos3 genotype.<h4>Methods</h4>Gene expression of Nos3 and platelet and endothelial cell adhesion molecule-1 (Pecam1) and NOS activity were measured in intima-containing media and adventitial specimens of ascending aortic tissue. The presence of 2 Nos3 single-nucleotide polymorphisms (SNPs; -786T/C and 894G/T) was determined for non-aneurysmal (NA) and aneurysmal patients with BAV (n = 40, 89, respectively); patients with tricuspid aortic valve (TAV) and aneurysm (n = 151); and NA patients with TAV (n = 100).<h4>Results</h4>Elevated Nos3 relative to Pecam1 and reduced Pecam1 relative to a housekeeping gene were obsEuropean journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic SurgeryLayer-specific Nos3 expression and genotypic distribution in bicuspid aortic valve aortopathy.PMC9615433HL109132R01 HL131632R01 HL109132HL131632Kotlarczyk MPPhillippi JARichards TDHill JCShiva SGleason TGBillaud MfalseLayer-specific Nos3 expression and genotypic distribution in bicuspid aortic valve aortopathy.<h4>Objectives</h4>We hypothesized that expression and activity of nitric oxide synthase-3 enzyme (Nos3) in bicuspid aortic valve (BAV) aortopathy are related to tissue layer and Nos3 genotype.<h4>Methods</h4>Gene expression of Nos3 and platelet and endothelial cell adhesion molecule-1 (Pecam1) and NOS activity were measured in intima-containing media and adventitial specimens of ascending aortic tissue. The presence of 2 Nos3 single-nucleotide polymorphisms (SNPs; -786T/C and 894G/T) was determined for non-aneurysmal (NA) and aneurysmal patients with BAV (n = 40, 89, respectively); patients with tricuspid aortic valve (TAV) and aneurysm (n = 151); and NA patients with TAV (n = 100).<h4>Results</h4>Elevated Nos3 relative to Pecam1 and reduced Pecam1 relative to a housekeeping gene were obs2022-01-01T00:00:00Z2022 Oct2026-04-08T12:08:53.065Z2025-02-19T04:15:52.464ZS-EPMC96154333546040310.1093/ejcts/ezac237