{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(5)"],"submitter":["Hu XY"],"pubmed_abstract":["The catalytic generation of H<sub>2</sub> in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru + Fe<sub>2</sub>S<sub>2</sub>@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [Fe<sub>2</sub>S<sub>2</sub>] sites, and photosensitizer Ru(bpy)<sub>3</sub> <sup>2+</sup>. Under blue light irradiation, hydrated protons were photochemically reduced to H<sub>2</sub>, which increased the local pH in living cells (HeLa cells). The generated H<sub>2</sub> was subsequently used as an antioxidant to decrease reactive oxygen species (ROS) levels in living cells (HEK 293T, HepG2, MCF-7, and HeLa cells). Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times, relative to that"],"journal":["Journal of pharmaceutical analysis"],"pagination":["801-807"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9615531"],"repository":["biostudies-literature"],"pubmed_title":["Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells."],"pmcid":["PMC9615531"],"pubmed_authors":["Yang ZW","Zhang J","Hu XY","Wang HS","Li JJ"],"additional_accession":[]},"is_claimable":false,"name":"Fluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells.","description":"The catalytic generation of H<sub>2</sub> in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru + Fe<sub>2</sub>S<sub>2</sub>@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [Fe<sub>2</sub>S<sub>2</sub>] sites, and photosensitizer Ru(bpy)<sub>3</sub> <sup>2+</sup>. Under blue light irradiation, hydrated protons were photochemically reduced to H<sub>2</sub>, which increased the local pH in living cells (HeLa cells). The generated H<sub>2</sub> was subsequently used as an antioxidant to decrease reactive oxygen species (ROS) levels in living cells (HEK 293T, HepG2, MCF-7, and HeLa cells). Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times, relative to that","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-05T13:27:31.953Z","creation":"2025-04-05T13:27:31.953Z"},"accession":"S-EPMC9615531","cross_references":{"pubmed":["36320605"],"doi":["10.1016/j.jpha.2022.05.007"]}}