biostudies-literatureUnknown12(5)Hu XYThe catalytic generation of H₂ in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru + Fe₂S₂@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [Fe₂S₂] sites, and photosensitizer Ru(bpy)₃ ²⁺. Under blue light irradiation, hydrated protons were photochemically reduced to H₂, which increased the local pH in living cells (HeLa cells). The generated H₂ was subsequently used as an antioxidant to decrease reactive oxygen species (ROS) levels in living cells (HEK 293T, HepG2, MCF-7, and HeLa cells). Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times, relative to thatJournal of pharmaceutical analysis801-807https://www.ebi.ac.uk/biostudies/studies/S-EPMC9615531biostudies-literatureFluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells.PMC9615531Yang ZWZhang JHu XYWang HSLi JJfalseFluorescent intracellular imaging of reactive oxygen species and pH levels moderated by a hydrogenase mimic in living cells.The catalytic generation of H₂ in living cells provides a method for antioxidant therapy. In this study, an [FeFe]-hydrogenase mimic [Ru + Fe₂S₂@F127(80)] was synthesized by self-assembling polymeric pluronic F-127, catalytic [Fe₂S₂] sites, and photosensitizer Ru(bpy)₃ ²⁺. Under blue light irradiation, hydrated protons were photochemically reduced to H₂, which increased the local pH in living cells (HeLa cells). The generated H₂ was subsequently used as an antioxidant to decrease reactive oxygen species (ROS) levels in living cells (HEK 293T, HepG2, MCF-7, and HeLa cells). Our findings revealed that the proliferation of HEK 293T cells increased by a factor of about six times, relative to that2022-01-01T00:00:00Z2022 Oct2025-04-05T13:27:31.953Z2025-04-05T13:27:31.953ZS-EPMC96155313632060510.1016/j.jpha.2022.05.007