{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ye H"],"funding":["Research Grants Council, University Grants Committee","National Natural Science Foundation of China"],"pagination":["e2205314119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9618059"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(43)"],"pubmed_abstract":["Autophagy is an intracellular degradation system for cytoplasmic constituents which is mediated by the formation of a double-membrane organelle termed the autophagosome and its subsequent fusion with the lysosome/vacuole. The formation of the autophagosome requires membrane from the endoplasmic reticulum (ER) and is tightly regulated by a series of autophagy-related (ATG) proteins and lipids. However, how the ER contacts autophagosomes and regulates autophagy remain elusive in plants. In this study, we identified and demonstrated the roles of <i>Arabidopsis</i> oxysterol-binding protein-related protein 2A (ORP2A) in mediating ER-autophagosomal membrane contacts and autophagosome biogenesis. We showed that ORP2A localizes to both ER-plasma membrane contact sites (EPCSs) and autophagosomes, "],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["<i>Arabidopsis</i> ORP2A mediates ER-autophagosomal membrane contact sites and regulates PI3P in plant autophagy."],"pmcid":["PMC9618059"],"funding_grant_id":["91854201","C4002-20W","C4033-19E","C2009-19G","CUHK14101219","C4041-18E","C4002-21EF","C4002-17G","AoE/M-05/12","R4005-18","CUHK14100818"],"pubmed_authors":["Huang S","Jiang L","Liang Z","Yu Q","Gao J","Lin Y","Ye H"],"additional_accession":[]},"is_claimable":false,"name":"<i>Arabidopsis</i> ORP2A mediates ER-autophagosomal membrane contact sites and regulates PI3P in plant autophagy.","description":"Autophagy is an intracellular degradation system for cytoplasmic constituents which is mediated by the formation of a double-membrane organelle termed the autophagosome and its subsequent fusion with the lysosome/vacuole. The formation of the autophagosome requires membrane from the endoplasmic reticulum (ER) and is tightly regulated by a series of autophagy-related (ATG) proteins and lipids. However, how the ER contacts autophagosomes and regulates autophagy remain elusive in plants. In this study, we identified and demonstrated the roles of <i>Arabidopsis</i> oxysterol-binding protein-related protein 2A (ORP2A) in mediating ER-autophagosomal membrane contacts and autophagosome biogenesis. We showed that ORP2A localizes to both ER-plasma membrane contact sites (EPCSs) and autophagosomes, ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-04T20:46:03.357Z","creation":"2025-04-04T20:46:03.357Z"},"accession":"S-EPMC9618059","cross_references":{"pubmed":["36252028"],"doi":["10.1073/pnas.2205314119"]}}