{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang C"],"funding":["US National Science Foundation","National Natural Science Foundation of China"],"pagination":["e2213540119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9618065"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(43)"],"pubmed_abstract":["Heterochromatin is essential for genomic integrity and stability in eukaryotes. The mechanisms that regulate meiotic heterochromatin formation remain largely undefined. Here, we show that the catalytic subunit (POL2A) of <i>Arabidopsis</i> DNA polymerase epsilon (POL ε) is required for proper formation of meiotic heterochromatin. The POL2A N terminus interacts with the GHKL adenosine triphosphatase (ATPase) MORC1 (Microrchidia 1), and POL2A is required for MORC1's localization on meiotic heterochromatin. Mutations affecting the POL2A N terminus cause aberrant morphology of meiotic heterochromatin, which is also observed in <i>morc1</i>. Moreover, the POL2A C-terminal zinc finger domain (ZF1) specifically binds to histone H3.1-H4 dimer or tetramer and is important for meiotic heterochromati"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["DNA polymerase epsilon binds histone H3.1-H4 and recruits MORC1 to mediate meiotic heterochromatin condensation."],"pmcid":["PMC9618065"],"funding_grant_id":["IOS-1844264","31870293","32000246","31925005"],"pubmed_authors":["Li Y","He C","Huang J","Zhang J","Li T","Wang C","Ma H","Jiang D","Copenhaver GP","Dong A","Wang Y"],"additional_accession":[]},"is_claimable":false,"name":"DNA polymerase epsilon binds histone H3.1-H4 and recruits MORC1 to mediate meiotic heterochromatin condensation.","description":"Heterochromatin is essential for genomic integrity and stability in eukaryotes. The mechanisms that regulate meiotic heterochromatin formation remain largely undefined. Here, we show that the catalytic subunit (POL2A) of <i>Arabidopsis</i> DNA polymerase epsilon (POL ε) is required for proper formation of meiotic heterochromatin. The POL2A N terminus interacts with the GHKL adenosine triphosphatase (ATPase) MORC1 (Microrchidia 1), and POL2A is required for MORC1's localization on meiotic heterochromatin. Mutations affecting the POL2A N terminus cause aberrant morphology of meiotic heterochromatin, which is also observed in <i>morc1</i>. Moreover, the POL2A C-terminal zinc finger domain (ZF1) specifically binds to histone H3.1-H4 dimer or tetramer and is important for meiotic heterochromati","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-04T08:29:12.535Z","creation":"2025-04-04T08:29:12.535Z"},"accession":"S-EPMC9618065","cross_references":{"pubmed":["36260743"],"doi":["10.1073/pnas.2213540119"]}}