{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mahamad Maifiah MH"],"funding":["Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","NIAID NIH HHS"],"pagination":["89"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9618192"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["29(1)"],"pubmed_abstract":["<h4>Background</h4>Understanding the mechanism of antimicrobial action is critical for improving antibiotic therapy. For the first time, we integrated correlative metabolomics and transcriptomics of Pseudomonas aeruginosa to elucidate the mechanism of synergistic killing of polymyxin-rifampicin combination.<h4>Methods</h4>Liquid chromatography-mass spectrometry and RNA-seq analyses were conducted to identify the significant changes in the metabolome and transcriptome of P. aeruginosa PAO1 after exposure to polymyxin B (1 mg/L) and rifampicin (2 mg/L) alone, or in combination over 24 h. A genome-scale metabolic network was employed for integrative analysis.<h4>Results</h4>In the first 4-h treatment, polymyxin B monotherapy induced significant lipid perturbations, predominantly to fatty acid"],"journal":["Journal of biomedical science"],"pubmed_title":["Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin-rifampicin combination against Pseudomonas aeruginosa."],"pmcid":["PMC9618192"],"funding_grant_id":["AI132154","R01 AI132681"],"pubmed_authors":["Tsuji BT","Li J","Velkov T","Zhu Y","Creek DJ","Mahamad Maifiah MH"],"additional_accession":[]},"is_claimable":false,"name":"Integrated metabolomic and transcriptomic analyses of the synergistic effect of polymyxin-rifampicin combination against Pseudomonas aeruginosa.","description":"<h4>Background</h4>Understanding the mechanism of antimicrobial action is critical for improving antibiotic therapy. For the first time, we integrated correlative metabolomics and transcriptomics of Pseudomonas aeruginosa to elucidate the mechanism of synergistic killing of polymyxin-rifampicin combination.<h4>Methods</h4>Liquid chromatography-mass spectrometry and RNA-seq analyses were conducted to identify the significant changes in the metabolome and transcriptome of P. aeruginosa PAO1 after exposure to polymyxin B (1 mg/L) and rifampicin (2 mg/L) alone, or in combination over 24 h. A genome-scale metabolic network was employed for integrative analysis.<h4>Results</h4>In the first 4-h treatment, polymyxin B monotherapy induced significant lipid perturbations, predominantly to fatty acid","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-18T16:55:42.725Z","creation":"2025-04-07T04:21:36.247Z"},"accession":"S-EPMC9618192","cross_references":{"pubmed":["36310165"],"doi":["10.1186/s12929-022-00874-3"]}}