{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(6)"],"submitter":["Khan MI"],"pubmed_abstract":["<h4>Introduction</h4><i>Moringa oleifera</i> is known as a 'natural nutrition of the tropics' because it provides vital nutritional supplements and a variety of pharmacological benefits. The focus of this study was to elucidate the dose dependent effects of <i>Moringa oleifera</i> leaf (MOL) extract on the growth of the human osteoblast-like osteosarcoma SaOS-2 cell line and primary osteoblast cells.<h4>Methods</h4>Trypan blue & tetrazolium assay, intracellular ROS generation, chromatin condensation, cell cycle analysis, alkaline phosphatase (ALP), mineralization, and osteogenic gene expression were tested on both treated and untreated osteosarcoma SaOS-2 cells.<h4>Results</h4>As revealed by cell viability assay, growth activity was observed at concentrations 25 and 50 μg/mL of MOL extract, whereas 100 and 200 μg/mL doses decreased the proliferation activity, resulting in ROS production and chromatin condensation. Cell cycle study revealed that MOL extract at 50 and 100 μg/mL concentrations arrested the cells in the G2/M phase. Low doses increased the ALP levels, mineralization, and expression of the bone morphogenetic protein 2 (BMP2) and runt-related transcription factor 2 (Runx2) genes in osteoblast-like SaOS-2 cells, however, high doses inhibited the proliferation properties of MOL extract. Through AutoDock Vina and iGEMDOCK 2.1, the interaction of active components of MOL, such as β-sitosterol, quercetin and kaempferol, with BMP2 and Runx2 proteins revealed a reasonable binding affinity. Moreover, these components did not show any Lipinski's rule of five violation and showed predictable pharmacokinetic properties.<h4>Conclusion</h4>The results of the biphasic dose-response of MOL extract on the growth activity of osteoblast-like SaOS-2 cells and <i>in silico</i> binding interface, may provide a therapeutic and/or preventive implication in prospective drug development."],"journal":["Journal of traditional and complementary medicine"],"pagination":["608-618"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9618397"],"repository":["biostudies-literature"],"pubmed_title":["Moringa oleifera leaf extract induces osteogenic-like differentiation of human osteosarcoma SaOS2 cells."],"pmcid":["PMC9618397"],"pubmed_authors":["Alanezi AA","Barkat HA","Arshad M","Siddiqui S","Khan MI","Barkat MA","Ashfaq F","Alhodieb FS"],"additional_accession":[]},"is_claimable":false,"name":"Moringa oleifera leaf extract induces osteogenic-like differentiation of human osteosarcoma SaOS2 cells.","description":"<h4>Introduction</h4><i>Moringa oleifera</i> is known as a 'natural nutrition of the tropics' because it provides vital nutritional supplements and a variety of pharmacological benefits. The focus of this study was to elucidate the dose dependent effects of <i>Moringa oleifera</i> leaf (MOL) extract on the growth of the human osteoblast-like osteosarcoma SaOS-2 cell line and primary osteoblast cells.<h4>Methods</h4>Trypan blue & tetrazolium assay, intracellular ROS generation, chromatin condensation, cell cycle analysis, alkaline phosphatase (ALP), mineralization, and osteogenic gene expression were tested on both treated and untreated osteosarcoma SaOS-2 cells.<h4>Results</h4>As revealed by cell viability assay, growth activity was observed at concentrations 25 and 50 μg/mL of MOL extract, whereas 100 and 200 μg/mL doses decreased the proliferation activity, resulting in ROS production and chromatin condensation. Cell cycle study revealed that MOL extract at 50 and 100 μg/mL concentrations arrested the cells in the G2/M phase. Low doses increased the ALP levels, mineralization, and expression of the bone morphogenetic protein 2 (BMP2) and runt-related transcription factor 2 (Runx2) genes in osteoblast-like SaOS-2 cells, however, high doses inhibited the proliferation properties of MOL extract. Through AutoDock Vina and iGEMDOCK 2.1, the interaction of active components of MOL, such as β-sitosterol, quercetin and kaempferol, with BMP2 and Runx2 proteins revealed a reasonable binding affinity. Moreover, these components did not show any Lipinski's rule of five violation and showed predictable pharmacokinetic properties.<h4>Conclusion</h4>The results of the biphasic dose-response of MOL extract on the growth activity of osteoblast-like SaOS-2 cells and <i>in silico</i> binding interface, may provide a therapeutic and/or preventive implication in prospective drug development.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-04T22:32:52.597Z","creation":"2025-02-19T03:23:55.131Z"},"accession":"S-EPMC9618397","cross_references":{"pubmed":["36325245"],"doi":["10.1016/j.jtcme.2022.08.006"]}}