biostudies-literatureMoon IMinistry of Science and ICTSoonchunhyang UniversityNational Research Foundation of Korea145-152https://www.ebi.ac.uk/biostudies/studies/S-EPMC9618905biostudies-literatureUnknown43(2)<h4>Background</h4>Clonal hematopoiesis of indeterminate potential (CHIP), which is defined as the presence of blood cells originating from somatically mutated hematopoietic stem cells, is common among the elderly and is associated with an increased risk of hematologic malignancies. We investigated the clinical, mutational, and transcriptomic characteristics in elderly Korean individuals with CHIP mutations.<h4>Methods</h4>We investigated CHIP in 90 elderly individuals aged ≥60 years with normal complete blood counts at a tertiary-care hospital in Korea between June 2021 and February 2022. Clinical and laboratory data were prospectively obtained. Targeted next-generation sequencing of 49 myeloid malignancy driver genes and massively parallel RNA sequencing were performed to explore the molecular spectrum and transcriptomic characteristics of CHIP mutations.<h4>Results</h4>We detected 51 mutations in 10 genes in 37 (41%) of the study individuals. CHIP prevalence increased with age. CHIP mutations were observed with high prevalence in <i>DNMT3A</i> (26 individuals) and <i>TET2</i> (eight individuals) and were also found in various other genes, including <i>KDM6A, SMC3, TP53, BRAF, PPM1D, SRSF2, STAG1</i>, and <i>ZRSR2</i>. Baseline characteristics, including age, confounding diseases, and blood cell parameters, showed no significant differences. Using mRNA sequencing, we characterized the altered gene expression profile, implicating neutrophil degranulation and innate immune system dysregulation.<h4>Conclusions</h4>Somatic CHIP driver mutations are common among the elderly in Korea and are detected in various genes, including <i>DNMT3A</i> and <i>TET2</i>. Our study highlights that chronic dysregulation of innate immune signaling is associated with the pathogenesis of various diseases, including hematologic malignancies.Annals of laboratory medicineClinical, Mutational, and Transcriptomic Characteristics in Elderly Korean Individuals With Clonal Hematopoiesis Driver Mutations.PMC96189052021R1C1C1005725Kim SHJang MAKong MGJi YSSuh JPark SKMoon IfalseClinical, Mutational, and Transcriptomic Characteristics in Elderly Korean Individuals With Clonal Hematopoiesis Driver Mutations.<h4>Background</h4>Clonal hematopoiesis of indeterminate potential (CHIP), which is defined as the presence of blood cells originating from somatically mutated hematopoietic stem cells, is common among the elderly and is associated with an increased risk of hematologic malignancies. We investigated the clinical, mutational, and transcriptomic characteristics in elderly Korean individuals with CHIP mutations.<h4>Methods</h4>We investigated CHIP in 90 elderly individuals aged ≥60 years with normal complete blood counts at a tertiary-care hospital in Korea between June 2021 and February 2022. Clinical and laboratory data were prospectively obtained. Targeted next-generation sequencing of 49 myeloid malignancy driver genes and massively parallel RNA sequencing were performed to explore the molecular spectrum and transcriptomic characteristics of CHIP mutations.<h4>Results</h4>We detected 51 mutations in 10 genes in 37 (41%) of the study individuals. CHIP prevalence increased with age. CHIP mutations were observed with high prevalence in <i>DNMT3A</i> (26 individuals) and <i>TET2</i> (eight individuals) and were also found in various other genes, including <i>KDM6A, SMC3, TP53, BRAF, PPM1D, SRSF2, STAG1</i>, and <i>ZRSR2</i>. Baseline characteristics, including age, confounding diseases, and blood cell parameters, showed no significant differences. Using mRNA sequencing, we characterized the altered gene expression profile, implicating neutrophil degranulation and innate immune system dysregulation.<h4>Conclusions</h4>Somatic CHIP driver mutations are common among the elderly in Korea and are detected in various genes, including <i>DNMT3A</i> and <i>TET2</i>. Our study highlights that chronic dysregulation of innate immune signaling is associated with the pathogenesis of various diseases, including hematologic malignancies.2023-01-01T00:00:00Z2023 Mar2024-11-13T02:24:03.898Z2024-11-13T02:24:03.898ZS-EPMC96189053628150810.3343/alm.2023.43.2.145