{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang J"],"funding":["Chinese Academy of Sciences","National Natural Science Foundation of China"],"pagination":["101789"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9619720"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(4)"],"pubmed_abstract":["We present a protocol for <i>Agrobacterium</i>-mediated genetic transformation and genome editing in wild allotetraploid rice <i>Oryza alta</i>. We detail steps to induce embryogenic calli from mature seeds and co-cultivate with <i>Agrobacterium</i> after infection. We further describe how to select transformed cells that proliferated from infected calli based on hygromycin resistance, resulting in regeneration of transformed plantlets with multiple edited alleles. Edited lines produced <i>via</i> this protocol can be used for gene functions studies and <i>de novo</i> domestication of <i>O. alta.</i> For complete details on the use and execution of this protocol, please refer to Yu et al. (2021)."],"journal":["STAR protocols"],"pubmed_title":["Protocol for genome editing in wild allotetraploid rice <i>Oryza alta</i>."],"pmcid":["PMC9619720"],"funding_grant_id":["32122064","YSBR-078","31788103","XDA24030504"],"pubmed_authors":["Zeng P","Li J","Zhang J","Yu H","Meng X"],"additional_accession":[]},"is_claimable":false,"name":"Protocol for genome editing in wild allotetraploid rice <i>Oryza alta</i>.","description":"We present a protocol for <i>Agrobacterium</i>-mediated genetic transformation and genome editing in wild allotetraploid rice <i>Oryza alta</i>. We detail steps to induce embryogenic calli from mature seeds and co-cultivate with <i>Agrobacterium</i> after infection. We further describe how to select transformed cells that proliferated from infected calli based on hygromycin resistance, resulting in regeneration of transformed plantlets with multiple edited alleles. Edited lines produced <i>via</i> this protocol can be used for gene functions studies and <i>de novo</i> domestication of <i>O. alta.</i> For complete details on the use and execution of this protocol, please refer to Yu et al. (2021).","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2026-05-10T05:13:14.394Z","creation":"2025-02-19T04:02:43.428Z"},"accession":"S-EPMC9619720","cross_references":{"pubmed":["36325580"],"doi":["10.1016/j.xpro.2022.101789"]}}