biostudies-literatureUnknown17(12)Christiansen SNChina Scholarship CouncilEllen and Aage Andersen’s FoundationNovo Nordic foundationThe Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring the DNA methylation of more than 850,000 CpG positions. In clinical and forensic settings, it is critical to be able to work with low DNA amounts without risking reduced reproducibility. We evaluated the EPIC for a range of DNA amounts using two-fold serial dilutions investigated on two different days. While the β-value distributions were generally unaffected by decreasing DNA amounts, the median squared Pearson's correlation coefficient (<i>R²</i>) of between-days β-value comparisons decreased from 0.994 (500 ng DNA) to 0.957 (16 ng DNA). The median standard deviation of the β-values was 0.005 and up to 0.017 (median of medians: 0.014) for β-values around 0.6-0.7. With decreasing amounts of DNA fromEpigenetics1636-1645https://www.ebi.ac.uk/biostudies/studies/S-EPMC9621037biostudies-literatureReproducibility of the Infinium methylationEPIC BeadChip assay using low DNA amounts.PMC9621037Morling NLiu JAndersen JDTfelt-Hansen JChristiansen SNKampmann MLAndersen MMfalseReproducibility of the Infinium methylationEPIC BeadChip assay using low DNA amounts.The Infinium MethylationEPIC BeadChip (EPIC) is a reliable method for measuring the DNA methylation of more than 850,000 CpG positions. In clinical and forensic settings, it is critical to be able to work with low DNA amounts without risking reduced reproducibility. We evaluated the EPIC for a range of DNA amounts using two-fold serial dilutions investigated on two different days. While the β-value distributions were generally unaffected by decreasing DNA amounts, the median squared Pearson's correlation coefficient (<i>R²</i>) of between-days β-value comparisons decreased from 0.994 (500 ng DNA) to 0.957 (16 ng DNA). The median standard deviation of the β-values was 0.005 and up to 0.017 (median of medians: 0.014) for β-values around 0.6-0.7. With decreasing amounts of DNA from2022-01-01T00:00:00Z2022 Dec2025-04-05T13:44:37.692Z2025-04-05T13:44:37.692ZS-EPMC96210373535686710.1080/15592294.2022.2051861