biostudies-literatureUnknown33(17)Pang Y<h4>Objective</h4>Epilepsy causes physical and mental damage to patients. As well known, microRNAs (miRNAs) provide therapeutic target potentials for patients with epilepsy. miR-128-3p was previously reported to be downregulated in temporal lobe epilepsy (TLE) patients, however, its detailed function in epilepsy is unknown.<h4>Methods</h4>Astrocytes function in epilepsy, penicillin-induced astrocytes can be used as a model for seizures in vitro. Currently, the expression levels of mitogen-activated protein kinase 6 (MAPK6), interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) were determined by western blot and reverse transcription-quantitative PCR analyses (RT-qPCR). The expression level of miR-128-3p was evaluated by RT-qPCR. TargetScan 7.1 and dual luciferase reporter assNeuroreport742-749https://www.ebi.ac.uk/biostudies/studies/S-EPMC9622368biostudies-literaturemiR-128-3p inhibits the inflammation by targeting MAPK6 in penicillin-induced astrocytes.PMC9622368Wang SPang YLuo DfalsemiR-128-3p inhibits the inflammation by targeting MAPK6 in penicillin-induced astrocytes.<h4>Objective</h4>Epilepsy causes physical and mental damage to patients. As well known, microRNAs (miRNAs) provide therapeutic target potentials for patients with epilepsy. miR-128-3p was previously reported to be downregulated in temporal lobe epilepsy (TLE) patients, however, its detailed function in epilepsy is unknown.<h4>Methods</h4>Astrocytes function in epilepsy, penicillin-induced astrocytes can be used as a model for seizures in vitro. Currently, the expression levels of mitogen-activated protein kinase 6 (MAPK6), interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) were determined by western blot and reverse transcription-quantitative PCR analyses (RT-qPCR). The expression level of miR-128-3p was evaluated by RT-qPCR. TargetScan 7.1 and dual luciferase reporter ass2022-01-01T00:00:00Z2022 Dec2025-04-04T21:24:17.643Z2025-04-04T21:24:17.643ZS-EPMC96223683625043710.1097/WNR.0000000000001840