biostudies-literatureUnknown43(11)Wang WDThe forkhead box M1 (FoxM1) protein, a transcription factor, plays critical roles in regulating tumor growth and drug resistance, while cellular FLICE-inhibitory protein (c-FLIP), an anti-apoptotic regulator, is involved in the ubiquitin-proteasome pathway. In this study, we investigated the effects of c-FLIP on the expression and ubiquitination levels of FoxM1 along with drug susceptibility in non-small-cell lung cancer (NSCLC) cells. We first showed that the expression levels of FoxM1 and c-FLIP were increased and positively correlated (R² = 0.1106, P < 0.0001) in 90 NSCLC samples. The survival data from prognostic analysis demonstrated that high expression of c-FLIP and/or FoxM1 was related to poor prognosis in NSCLC patients and that the combination of FoxM1 and c-FLIP couldActa pharmacologica Sinica2956-2966https://www.ebi.ac.uk/biostudies/studies/S-EPMC9622852biostudies-literaturec-FLIP promotes drug resistance in non-small-cell lung cancer cells via upregulating FoxM1 expression.PMC9622852Ni JChen SZWang CWang AMShang YLi GJSu LWang WDfalsec-FLIP promotes drug resistance in non-small-cell lung cancer cells via upregulating FoxM1 expression.The forkhead box M1 (FoxM1) protein, a transcription factor, plays critical roles in regulating tumor growth and drug resistance, while cellular FLICE-inhibitory protein (c-FLIP), an anti-apoptotic regulator, is involved in the ubiquitin-proteasome pathway. In this study, we investigated the effects of c-FLIP on the expression and ubiquitination levels of FoxM1 along with drug susceptibility in non-small-cell lung cancer (NSCLC) cells. We first showed that the expression levels of FoxM1 and c-FLIP were increased and positively correlated (R² = 0.1106, P < 0.0001) in 90 NSCLC samples. The survival data from prognostic analysis demonstrated that high expression of c-FLIP and/or FoxM1 was related to poor prognosis in NSCLC patients and that the combination of FoxM1 and c-FLIP could2022-01-01T00:00:00Z2022 Nov2025-05-29T21:44:19.8Z2025-05-29T21:44:19.8ZS-EPMC96228523542208510.1038/s41401-022-00905-7