biostudies-literatureUnknown13Bhattacharyya P<i>Malaxis acuminata</i> D. Don [=<i>Crepidium acuminatum</i> (D. Don) Szlach.] is an endangered medicinal orchid of the <i>Ashtvarga</i> group of plants in <i>Ayurveda</i> (Indian system of traditional medicine). Using a combination of aromatic cytokinin [<i>meta</i>-Topolin (<i>m</i>T)], plant biostimulant (chitosan), auxin [indole-3-butyric acid (IBA)], and a phenolic elicitor [phloroglucinol (PG)], plants of <i>M. acuminata</i> were regenerated <i>in vitro</i> for mass multiplication. The present research reveals the first-ever transcriptome of <i>M. acuminata</i>. A total of 43,111 transcripts encoding 23,951 unigenes were assembled <i>de novo</i> from a total of 815.02 million reads obtained from leaf and pseudobulb of <i>in vitro</i> raised <i>M. acuminata.</i> Expression analysis oFrontiers in plant science954467https://www.ebi.ac.uk/biostudies/studies/S-EPMC9623264biostudies-literature<i>De novo</i> transcriptome based insights into secondary metabolite biosynthesis in <i>Malaxis acuminata</i> (<i>Jeevak</i>)-A therapeutically important orchid.PMC9623264Shankar RSharma TSwarnkar MKBhattacharyya PYadav AJoshi RRituLalthafamkimi LKumar Sfalse<i>De novo</i> transcriptome based insights into secondary metabolite biosynthesis in <i>Malaxis acuminata</i> (<i>Jeevak</i>)-A therapeutically important orchid.<i>Malaxis acuminata</i> D. Don [=<i>Crepidium acuminatum</i> (D. Don) Szlach.] is an endangered medicinal orchid of the <i>Ashtvarga</i> group of plants in <i>Ayurveda</i> (Indian system of traditional medicine). Using a combination of aromatic cytokinin [<i>meta</i>-Topolin (<i>m</i>T)], plant biostimulant (chitosan), auxin [indole-3-butyric acid (IBA)], and a phenolic elicitor [phloroglucinol (PG)], plants of <i>M. acuminata</i> were regenerated <i>in vitro</i> for mass multiplication. The present research reveals the first-ever transcriptome of <i>M. acuminata</i>. A total of 43,111 transcripts encoding 23,951 unigenes were assembled <i>de novo</i> from a total of 815.02 million reads obtained from leaf and pseudobulb of <i>in vitro</i> raised <i>M. acuminata.</i> Expression analysis o2022-01-01T00:00:00Z20222026-05-03T10:52:50.168Z2025-04-19T02:35:46.76ZS-EPMC96232643633025710.3389/fpls.2022.954467