biostudies-literatureZhu XExpert Workstation for Jingcheng Dong in Yunnan Province6842530https://www.ebi.ac.uk/biostudies/studies/S-EPMC9626231biostudies-literatureUnknown2022<h4>Background</h4>Cycloastragenol (CAG) has been reported to alleviate airway inflammation in ovalbumin- (OVA-) induced asthmatic mice. However, its specific mechanisms remain unclear.<h4>Objective</h4>This study is aimed at investigating the effects of CAG on asthma, comparing its efficacy with dexamethasone (DEX), and elucidating the mechanism of CAG's regulation.<h4>Methods</h4>The asthma mouse model was induced by OVA. CAG at the optimal dose of 125 mg/kg was given every day from day 0 for 20-day prevention or from day 14 for a 7-day treatment. We observed the preventive and therapeutic effects of CAG in asthmatic mice by evaluating the airway inflammation, AHR, and mucus secretion. Lung proteins were used for TMT-based quantitative proteomic analysis to enunciate its regulatory mechaOxidative medicine and cellular longevityTMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice.PMC96262318217417021S2190250020210101Ruan ZLi CChen MZhu HZhou YWei YYu HHuang XDong JZhu XLiu BXu WShi HSun JfalseTMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice.<h4>Background</h4>Cycloastragenol (CAG) has been reported to alleviate airway inflammation in ovalbumin- (OVA-) induced asthmatic mice. However, its specific mechanisms remain unclear.<h4>Objective</h4>This study is aimed at investigating the effects of CAG on asthma, comparing its efficacy with dexamethasone (DEX), and elucidating the mechanism of CAG's regulation.<h4>Methods</h4>The asthma mouse model was induced by OVA. CAG at the optimal dose of 125 mg/kg was given every day from day 0 for 20-day prevention or from day 14 for a 7-day treatment. We observed the preventive and therapeutic effects of CAG in asthmatic mice by evaluating the airway inflammation, AHR, and mucus secretion. Lung proteins were used for TMT-based quantitative proteomic analysis to enunciate its regulatory mecha2022-01-01T00:00:00Z20222026-05-28T21:27:01.179Z2025-04-05T14:54:29.781ZS-EPMC96262313632980010.1155/2022/6842530