biostudies-literatureChoi HJRural Development AdministrationMinistry of Agriculture, Food and Rural AffairsKorea Smart Farm R&amp;D FoundationMinistry of Science and ICT, South KoreaNational Research Foundation of KoreaChonnam National UniversityKorea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry117-125https://www.ebi.ac.uk/biostudies/studies/S-EPMC9628822biostudies-literatureUnknown32(1)Until recently, four types of cellobiose-fermenting <i>Saccharomyces cerevisiae</i> strains have been developed by introduction of a cellobiose metabolic pathway based on either intracellular β-glucosidase (GH1-1) or cellobiose phosphorylase (CBP), along with either an energy-consuming active cellodextrin transporter (CDT-1) or a non-energy-consuming passive cellodextrin facilitator (CDT-2). In this study, the ethanol production performance of two cellobiose-fermenting <i>S. cerevisiae</i> strains expressing mutant CDT-2 (N306I) with GH1-1 or CBP were compared with two cellobiose-fermenting <i>S. cerevisiae</i> strains expressing mutant CDT-1 (F213L) with GH1-1 or CBP in the simultaneous saccharification and fermentation (SSF) of cellulose under various conditions. It was found that, regarJournal of microbiology and biotechnologyEffects of Engineered <i>Saccharomyces cerevisiae</i> Fermenting Cellobiose through Low-Energy-Consuming Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation.PMC9628822421045-032020-3893NRF-2019R1F1A1059516Choi HJJin YSLee WHfalseEffects of Engineered <i>Saccharomyces cerevisiae</i> Fermenting Cellobiose through Low-Energy-Consuming Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation.Until recently, four types of cellobiose-fermenting <i>Saccharomyces cerevisiae</i> strains have been developed by introduction of a cellobiose metabolic pathway based on either intracellular β-glucosidase (GH1-1) or cellobiose phosphorylase (CBP), along with either an energy-consuming active cellodextrin transporter (CDT-1) or a non-energy-consuming passive cellodextrin facilitator (CDT-2). In this study, the ethanol production performance of two cellobiose-fermenting <i>S. cerevisiae</i> strains expressing mutant CDT-2 (N306I) with GH1-1 or CBP were compared with two cellobiose-fermenting <i>S. cerevisiae</i> strains expressing mutant CDT-1 (F213L) with GH1-1 or CBP in the simultaneous saccharification and fermentation (SSF) of cellulose under various conditions. It was found that, regar2022-01-01T00:00:00Z2022 Jan2025-04-04T22:14:57.963Z2025-04-04T22:14:57.963ZS-EPMC96288223494975110.4014/jmb.2111.11047