{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zeng J"],"funding":["China Postdoctoral Science Foundation","National Natural Sciences Foundation of China","Chongqing Postdoctoral Science Foundation","China Postdoctoral Science Special Foundation"],"pagination":["6758-6772"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9629787"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["73(19)"],"pubmed_abstract":["Cytokinin is considered to be an important driver of seed yield. To increase the yield of cotton while avoiding the negative consequences caused by constitutive overproduction of cytokinin, we down-regulated specifically the carpel genes for cytokinin oxidase/dehydrogenase (CKX), a key negative regulator of cytokinin levels, in transgenic cotton. The carpel-specific down-regulation of CKXs significantly enhanced cytokinin levels in the carpels. The elevated cytokinin promoted the expression of carpel- and ovule-development-associated genes, GhSTK2, GhAG1, and GhSHP, boosting ovule formation and thus producing more seeds in the ovary. Field experiments showed that the carpel-specific increase of cytokinin significantly increased both seed yield and fiber yield of cotton, without resulting i"],"journal":["Journal of experimental botany"],"pubmed_title":["Carpel-specific down-regulation of GhCKXs in cotton significantly enhances seed and fiber yield."],"pmcid":["PMC9629787"],"funding_grant_id":["2021T140569","cstc2020jcyj-bshX0019","U2003209","31130039","2020M673104"],"pubmed_authors":["Liu H","Ding B","Zhang J","Xiao Y","Wang F","Zhang M","Liu R","Yan X","Bai W","Hou L","Ren H","Li X","Ding X","Zeng J","Chen Y","Pei Y","Zhao J"],"additional_accession":[]},"is_claimable":false,"name":"Carpel-specific down-regulation of GhCKXs in cotton significantly enhances seed and fiber yield.","description":"Cytokinin is considered to be an important driver of seed yield. To increase the yield of cotton while avoiding the negative consequences caused by constitutive overproduction of cytokinin, we down-regulated specifically the carpel genes for cytokinin oxidase/dehydrogenase (CKX), a key negative regulator of cytokinin levels, in transgenic cotton. The carpel-specific down-regulation of CKXs significantly enhanced cytokinin levels in the carpels. The elevated cytokinin promoted the expression of carpel- and ovule-development-associated genes, GhSTK2, GhAG1, and GhSHP, boosting ovule formation and thus producing more seeds in the ovary. Field experiments showed that the carpel-specific increase of cytokinin significantly increased both seed yield and fiber yield of cotton, without resulting i","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-04T07:45:32.136Z","creation":"2025-04-04T07:45:32.136Z"},"accession":"S-EPMC9629787","cross_references":{"pubmed":["35792654"],"doi":["10.1093/jxb/erac303"]}}