biostudies-literatureKrogvold LThe Novo Nordisk FoundationItalian Ministry of HealthSouth-Eastern Norway Regional Health AuthorityMedical Research CouncilEuropean Union&apos;s Seventh Framework ProgrammeJuvenile Diabetes Research Foundation2108-2120https://www.ebi.ac.uk/biostudies/studies/S-EPMC9630231biostudies-literatureUnknown65(12)<h4>Aims/hypothesis</h4>Enterovirus (EV) infection of pancreatic islet cells is one possible factor contributing to type 1 diabetes development. We have reported the presence of EV genome by PCR and of EV proteins by immunohistochemistry in pancreatic sections. Here we explore multiple human virus species in the Diabetes Virus Detection (DiViD) study cases using innovative methods, including virus passage in cell cultures.<h4>Methods</h4>Six recent-onset type 1 diabetes patients (age 24-35) were included in the DiViD study. Minimal pancreatic tail resection was performed under sterile conditions. Eleven live cases (age 43-83) of pancreatic carcinoma without diabetes served as control cases. In the present study, we used EV detection methods that combine virus growth in cell culture, gene aDiabetologiaLive enteroviruses, but not other viruses, detected in human pancreas at the onset of type 1 diabetes in the DiViD study.PMC96302315-CDA-2014-221-A-NFP7/2007-2013JDRF grant 25-2012-770PE-2013-02357094MR/P010695/1Krogvold LCampani DGenoni APuggioni ARichardson SJFlaxman CSBuanes TDahl-Jorgensen KToniolo AEdwin BfalseLive enteroviruses, but not other viruses, detected in human pancreas at the onset of type 1 diabetes in the DiViD study.<h4>Aims/hypothesis</h4>Enterovirus (EV) infection of pancreatic islet cells is one possible factor contributing to type 1 diabetes development. We have reported the presence of EV genome by PCR and of EV proteins by immunohistochemistry in pancreatic sections. Here we explore multiple human virus species in the Diabetes Virus Detection (DiViD) study cases using innovative methods, including virus passage in cell cultures.<h4>Methods</h4>Six recent-onset type 1 diabetes patients (age 24-35) were included in the DiViD study. Minimal pancreatic tail resection was performed under sterile conditions. Eleven live cases (age 43-83) of pancreatic carcinoma without diabetes served as control cases. In the present study, we used EV detection methods that combine virus growth in cell culture, gene a2022-01-01T00:00:00Z2022 Dec2026-05-03T10:35:15.697Z2024-11-21T02:28:57.201ZS-EPMC96302313595372710.1007/s00125-022-05779-2