{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zeng C"],"funding":["Natural Scientific Foundation of Guangdong Province","Scientific ResearchStar plan of Shunde Hospital"],"pagination":["3766-3775"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9633309"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["113(11)"],"pubmed_abstract":["SHARPIN is a tumor-associated gene involved in the growth and proliferation of many tumor types. A function of SHARPIN in cholangiocarcinoma (CCA) is so far unclear. Here, we studied the role and function of SHARPIN in CCA and revealed its relevant molecular mechanism. The expression of SHARPIN was analyzed in cholangiocarcinoma tissues from patients using immunohistochemistry, quantitative PCR, and western blot analysis. Expression of SHARPIN was suppressed/overexpressed by siRNA silencing or lentiviral overexpression vector, and the effect on cell proliferation was determined by the CCK-8 assay and flow cytometry. Accumulation of reactive oxygen species was measured with MitoTracker, and JC-1 staining showed mitochondrial fission/fusion and mitochondrial membrane potential changes as a r"],"journal":["Cancer science"],"pubmed_title":["SHARPIN promotes cell proliferation of cholangiocarcinoma and inhibits ferroptosis via p53/SLC7A11/GPX4 signaling."],"pmcid":["PMC9633309"],"funding_grant_id":["2021A1515010928","SRSP2019012","SRSP2021003","SRSP2021005","SRSP2021016"],"pubmed_authors":["Zeng C","Shen K","Yao J","Zhang K","Dong X","Zeng L","Liu Q","Zeng X","Wang W","Lin J","Ou H","Wei Z"],"additional_accession":[]},"is_claimable":false,"name":"SHARPIN promotes cell proliferation of cholangiocarcinoma and inhibits ferroptosis via p53/SLC7A11/GPX4 signaling.","description":"SHARPIN is a tumor-associated gene involved in the growth and proliferation of many tumor types. A function of SHARPIN in cholangiocarcinoma (CCA) is so far unclear. Here, we studied the role and function of SHARPIN in CCA and revealed its relevant molecular mechanism. The expression of SHARPIN was analyzed in cholangiocarcinoma tissues from patients using immunohistochemistry, quantitative PCR, and western blot analysis. Expression of SHARPIN was suppressed/overexpressed by siRNA silencing or lentiviral overexpression vector, and the effect on cell proliferation was determined by the CCK-8 assay and flow cytometry. Accumulation of reactive oxygen species was measured with MitoTracker, and JC-1 staining showed mitochondrial fission/fusion and mitochondrial membrane potential changes as a r","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-03T22:23:02.668Z","creation":"2025-02-19T00:31:41.114Z"},"accession":"S-EPMC9633309","cross_references":{"pubmed":["35968603"],"doi":["10.1111/cas.15531"]}}