biostudies-literatureKim DSNIDDK NIH HHSSusan G. KomenU.S. Department of Defense -BCRPNCI NIH HHSCancer Prevention and Research Institute of TexasNIH HHS1623-1635https://www.ebi.ac.uk/biostudies/studies/S-EPMC9633386biostudies-literatureUnknown20(11)Long noncoding RNAs have been implicated in many of the hallmarks of cancer. Herein, we found that the expression of lncRNA152 (lnc152; a.k.a. DRAIC), which we annotated previously, is highly upregulated in luminal breast cancer (LBC) and downregulated in triple-negative breast cancer (TNBC). Knockdown of lnc152 promotes cell migration and invasion in LBC cell lines. In contrast, ectopic expression of lnc152 inhibits growth, migration, invasion, and angiogenesis in TNBC cell lines. In mice, lnc152 inhibited the growth of TNBC cell xenografts, as well as metastasis of TNBC cells in an intracardiac injection model. Transcriptome analysis of the xenografts indicated that lnc152 downregulates genes controlling angiogenesis. Using pull down assays followed by LC/MS-MS, we identified RBM47, a knMolecular cancer research : MCRFunctional Characterization of lncRNA152 as an Angiogenesis-Inhibiting Tumor Suppressor in Triple-Negative Breast Cancers.PMC9633386S10 OD021684RP190235RP160318R01 DK069710RP130607PDF230441BC134066P30 CA142543Camacho CVKim DSKraus WLKim KHou TYNandu TGadad SSMalladi SSetlem RfalseFunctional Characterization of lncRNA152 as an Angiogenesis-Inhibiting Tumor Suppressor in Triple-Negative Breast Cancers.Long noncoding RNAs have been implicated in many of the hallmarks of cancer. Herein, we found that the expression of lncRNA152 (lnc152; a.k.a. DRAIC), which we annotated previously, is highly upregulated in luminal breast cancer (LBC) and downregulated in triple-negative breast cancer (TNBC). Knockdown of lnc152 promotes cell migration and invasion in LBC cell lines. In contrast, ectopic expression of lnc152 inhibits growth, migration, invasion, and angiogenesis in TNBC cell lines. In mice, lnc152 inhibited the growth of TNBC cell xenografts, as well as metastasis of TNBC cells in an intracardiac injection model. Transcriptome analysis of the xenografts indicated that lnc152 downregulates genes controlling angiogenesis. Using pull down assays followed by LC/MS-MS, we identified RBM47, a kn2022-01-01T00:00:00Z2022 Nov2026-05-27T10:53:55.889Z2024-11-20T02:13:49.764ZS-EPMC96333863599763510.1158/1541-7786.mcr-22-012310.1158/1541-7786.MCR-22-0123