{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Karasawa A"],"funding":["National Institutes of Health, National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["768-777"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9634608"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(Pt 6)"],"pubmed_abstract":["<i>De novo</i> structure determination from single-wavelength anomalous diffraction using native sulfur or phospho-rus in biomolecules (native-SAD) is an appealing method to mitigate the labor-intensive production of heavy-atom derivatives and seleno-methio-nyl substitutions. The native-SAD method is particularly attractive for membrane proteins, which are difficult to produce and often recalcitrant to grow into decent-sized crystals. Native-SAD uses lower-energy X-rays to enhance anomalous signals from sulfur or phospho-rus. However, at lower energies, the scattering and absorption of air contribute to the background noise, reduce the signals and are thus adverse to native-SAD phasing. We have previously demonstrated native-SAD phasing at an energy of 5 keV in air at the NSLS-II FMX beaml"],"journal":["IUCrJ"],"pubmed_title":["Multi-crystal native-SAD phasing at 5 keV with a helium environment."],"pmcid":["PMC9634608"],"funding_grant_id":["107462","P30 GM133893","116799","R01 GM107462","P41 GM116799"],"pubmed_authors":["McSweeney S","Hendrickson WA","Shi W","Liu Q","Andi B","Karasawa A","Fuchs MR"],"additional_accession":[]},"is_claimable":false,"name":"Multi-crystal native-SAD phasing at 5 keV with a helium environment.","description":"<i>De novo</i> structure determination from single-wavelength anomalous diffraction using native sulfur or phospho-rus in biomolecules (native-SAD) is an appealing method to mitigate the labor-intensive production of heavy-atom derivatives and seleno-methio-nyl substitutions. The native-SAD method is particularly attractive for membrane proteins, which are difficult to produce and often recalcitrant to grow into decent-sized crystals. Native-SAD uses lower-energy X-rays to enhance anomalous signals from sulfur or phospho-rus. However, at lower energies, the scattering and absorption of air contribute to the background noise, reduce the signals and are thus adverse to native-SAD phasing. We have previously demonstrated native-SAD phasing at an energy of 5 keV in air at the NSLS-II FMX beaml","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-05-27T11:16:45.591Z","creation":"2025-04-05T19:13:23.236Z"},"accession":"S-EPMC9634608","cross_references":{"pubmed":["36381147"],"doi":["10.1107/S205225252200971X"]}}