biostudies-literatureZhou XCamille and Henry Dreyfus FoundationUniversity of VirginiaNational Institute of General Medical SciencesNIGMS NIH HHS11941-11944https://www.ebi.ac.uk/biostudies/studies/S-EPMC9634815biostudies-literatureUnknown58(85)Photoacoustic (PA) imaging is a powerful biomedical imaging modality. We designed KeTMR and KeJuR, two xanthene-based dyes that were readily obtained through a 2-step synthetic route. KeJuR has low molecular weight, good aqueous solubility, and superior chemical stability compared to KeTMR. KeJuR shows a robust PA signal under 860 nm excitation and can be paired with traditional PA dyes for multiplex imaging in blood samples under a tissue-mimicking environment.Chemical communications (Cambridge, England)A long-wavelength xanthene dye for photoacoustic imaging.PMC9634815R35119751R35 GM119855R35 GM119751R35119855Fang YMiller EWWimalasiri VStains CIZhou XfalseA long-wavelength xanthene dye for photoacoustic imaging.Photoacoustic (PA) imaging is a powerful biomedical imaging modality. We designed KeTMR and KeJuR, two xanthene-based dyes that were readily obtained through a 2-step synthetic route. KeJuR has low molecular weight, good aqueous solubility, and superior chemical stability compared to KeTMR. KeJuR shows a robust PA signal under 860 nm excitation and can be paired with traditional PA dyes for multiplex imaging in blood samples under a tissue-mimicking environment.2022-01-01T00:00:00Z2022 Oct2025-04-22T11:51:23.252Z2025-04-06T00:09:31.405ZS-EPMC96348153619695710.1039/d2cc03947h