{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Stenvall CA"],"funding":["NIGMS NIH HHS"],"pagination":["ar121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9634972"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["33(13)"],"pubmed_abstract":["Keratin intermediate filaments convey mechanical stability and protection against stress to epithelial cells. Keratins are essential for colon health, as seen in keratin 8 knockout (K8<sup>-/-</sup>) mice exhibiting a colitis phenotype. We hypothesized that keratins support the nuclear envelope and lamina in colonocytes. K8<sup>-/-</sup> colonocytes in vivo exhibit significantly decreased levels of lamins A/C, B1, and B2 in a colon-specific and cell-intrinsic manner. CRISPR/Cas9- or siRNA-mediated K8 knockdown in Caco-2 cells similarly decreased lamin levels, which recovered after reexpression of K8 following siRNA treatment. Nuclear area was not decreased, and roundness was only marginally increased in cells without K8. Down-regulation of K8 in adult K8<sup>flox/flox</sup>;Villin-CreER<su"],"journal":["Molecular biology of the cell"],"pubmed_title":["Cytoplasmic keratins couple with and maintain nuclear envelope integrity in colonic epithelial cells."],"pmcid":["PMC9634972"],"funding_grant_id":["P01 GM096971"],"pubmed_authors":["Nystrom JH","Heikkila TRH","Toivola DM","Jansson T","Foisner R","Goldman RD","Ridge KM","Butler-Hallissey C","Adam SA","Stenvall CA"],"additional_accession":[]},"is_claimable":false,"name":"Cytoplasmic keratins couple with and maintain nuclear envelope integrity in colonic epithelial cells.","description":"Keratin intermediate filaments convey mechanical stability and protection against stress to epithelial cells. Keratins are essential for colon health, as seen in keratin 8 knockout (K8<sup>-/-</sup>) mice exhibiting a colitis phenotype. We hypothesized that keratins support the nuclear envelope and lamina in colonocytes. K8<sup>-/-</sup> colonocytes in vivo exhibit significantly decreased levels of lamins A/C, B1, and B2 in a colon-specific and cell-intrinsic manner. CRISPR/Cas9- or siRNA-mediated K8 knockdown in Caco-2 cells similarly decreased lamin levels, which recovered after reexpression of K8 following siRNA treatment. Nuclear area was not decreased, and roundness was only marginally increased in cells without K8. Down-regulation of K8 in adult K8<sup>flox/flox</sup>;Villin-CreER<su","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-21T14:17:03.948Z","creation":"2025-04-21T14:17:03.948Z"},"accession":"S-EPMC9634972","cross_references":{"pubmed":["36001365"],"doi":["10.1091/mbc.E20-06-0387"]}}