biostudies-literatureLi ZSpecial Program for Clinical Research in Health Industry of Shanghai Municipal Health Commission61https://www.ebi.ac.uk/biostudies/studies/S-EPMC9636808biostudies-literatureUnknown23(1)<h4>Background</h4>In brain, microvascular endothelial cells are exposed to various forces, including shear stress (SS). However, little is known about the effects of high shear stress (HSS) on human brain microvascular endothelial cells (HBMECs) and the underlying mechanism. The cholesterol efflux regulator ATP-binding cassette subfamily A member 1 (ABCA1) has been demonstrated to exert protective effect on HBMECs. However, whether ABCA1 is involved in the mechanism underneath the effect of HSS on HBMECs remains obscure. In the present study, a series of experiments were performed to better understand the effect of HSS on cellular processes of HBMECs and the possible involvement of ABCA1 and PI3K/Akt/eNOS in the underlying mechanisms.<h4>Results</h4>HBMECs were subjected to physiological BMC neuroscienceCholesterol efflux regulator ABCA1 exerts protective role against high shear stress-induced injury of HBMECs via regulating PI3K/Akt/eNOS signaling.PMC9636808YW-BD-0166-001Zhou LHLi ZLi JNLi QLiu CZhang QXu YfalseCholesterol efflux regulator ABCA1 exerts protective role against high shear stress-induced injury of HBMECs via regulating PI3K/Akt/eNOS signaling.<h4>Background</h4>In brain, microvascular endothelial cells are exposed to various forces, including shear stress (SS). However, little is known about the effects of high shear stress (HSS) on human brain microvascular endothelial cells (HBMECs) and the underlying mechanism. The cholesterol efflux regulator ATP-binding cassette subfamily A member 1 (ABCA1) has been demonstrated to exert protective effect on HBMECs. However, whether ABCA1 is involved in the mechanism underneath the effect of HSS on HBMECs remains obscure. In the present study, a series of experiments were performed to better understand the effect of HSS on cellular processes of HBMECs and the possible involvement of ABCA1 and PI3K/Akt/eNOS in the underlying mechanisms.<h4>Results</h4>HBMECs were subjected to physiological 2022-01-01T00:00:00Z2022 Nov2025-05-31T22:57:17.113Z2025-05-31T22:57:17.113ZS-EPMC96368083633530110.1186/s12868-022-00748-2