{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sa da Bandeira D"],"funding":["British Heart Foundation","Medical Research Council","Wellcome Trust","Academy of Medical Sciences"],"pagination":["111114"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9638014"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(3)"],"pubmed_abstract":["Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ<sup>+</sup> cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ<sup>+</sup> embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro."],"journal":["Cell reports"],"pubmed_title":["PDGFRβ<sup>+</sup> cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny."],"pmcid":["PMC9638014"],"funding_grant_id":["219542/Z/19/Z","RE/18/5/34216","SBF001\\1007","RM/17/3/33381"],"pubmed_authors":["Rossi F","van der Linden R","Sa da Bandeira D","Vink CS","Beltran M","Vermeren M","Henderson NC","Gonzalez ZN","Jung B","van de Werken HJG","Forbes SJ","Kilpatrick AM","Stefancova D","van Ijcken WFJ","Crisan M","Ventura T","Gomez-Salazar M","Marques M","Betsholtz C","Cuervo H"],"additional_accession":[]},"is_claimable":false,"name":"PDGFRβ<sup>+</sup> cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny.","description":"Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ<sup>+</sup> cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ<sup>+</sup> embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jul","modification":"2024-11-10T02:22:39.247Z","creation":"2024-11-10T02:22:39.247Z"},"accession":"S-EPMC9638014","cross_references":{"pubmed":["35858557"],"doi":["10.1016/j.celrep.2022.111114"]}}