biostudies-literatureSa da Bandeira DBritish Heart FoundationMedical Research CouncilWellcome TrustAcademy of Medical Sciences111114https://www.ebi.ac.uk/biostudies/studies/S-EPMC9638014biostudies-literatureUnknown40(3)Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ⁺ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ⁺ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.Cell reportsPDGFRβ⁺ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny.PMC9638014219542/Z/19/ZRE/18/5/34216SBF001\1007RM/17/3/33381Rossi Fvan der Linden RSa da Bandeira DVink CSBeltran MVermeren MHenderson NCGonzalez ZNJung Bvan de Werken HJGForbes SJKilpatrick AMStefancova Dvan Ijcken WFJCrisan MVentura TGomez-Salazar MMarques MBetsholtz CCuervo HfalsePDGFRβ⁺ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny.Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ⁺ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ⁺ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.2022-01-01T00:00:00Z2022 Jul2024-11-10T02:22:39.247Z2024-11-10T02:22:39.247ZS-EPMC96380143585855710.1016/j.celrep.2022.111114