{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bonaventure B"],"funding":["Institut des sciences biologiques","European Research Council","Agence Nationale de la Recherche","Agence Nationale de Recherches sur le Sida et les Hépatites Virales","Institut National de la Santé et de la Recherche Médicale","Université de Montpellier","Fondation pour la Recherche Médicale"],"pagination":["e54061"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9638865"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(11)"],"pubmed_abstract":["Genome-wide screens are powerful approaches to unravel regulators of viral infections. Here, a CRISPR screen identifies the RNA helicase DDX42 as an intrinsic antiviral inhibitor of HIV-1. Depletion of endogenous DDX42 increases HIV-1 DNA accumulation and infection in cell lines and primary cells. DDX42 overexpression inhibits HIV-1 infection, whereas expression of a dominant-negative mutant increases infection. Importantly, DDX42 also restricts LINE-1 retrotransposition and infection with other retroviruses and positive-strand RNA viruses, including CHIKV and SARS-CoV-2. However, DDX42 does not impact the replication of several negative-strand RNA viruses, arguing against an unspecific effect on target cells, which is confirmed by RNA-seq analysis. Proximity ligation assays show DDX42 in "],"journal":["EMBO reports"],"pubmed_title":["The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses."],"pmcid":["PMC9638865"],"funding_grant_id":["FDT202106013175","FDT201904008024","ANR‐10‐INBS‐09","ECTZ35478","ECTZ21792","759226"],"pubmed_authors":["Lacroix L","Rebendenne A","Djilli W","Moncorge O","Jouvenet N","McKellar J","Lavigne M","Arnaud-Arnould M","Rialle S","Ricci EP","Bonaventure B","Schulz R","Chaves Valadao AL","Gracias S","Blaise M","Parrinello H","Goujon C","Paillart JC","Briant L","Courgnaud V","Tauziet M","Labaronne E","Garcia de Gracia F","Vivet-Boudou V","Bernard E","Gros N"],"additional_accession":[]},"is_claimable":false,"name":"The DEAD box RNA helicase DDX42 is an intrinsic inhibitor of positive-strand RNA viruses.","description":"Genome-wide screens are powerful approaches to unravel regulators of viral infections. Here, a CRISPR screen identifies the RNA helicase DDX42 as an intrinsic antiviral inhibitor of HIV-1. Depletion of endogenous DDX42 increases HIV-1 DNA accumulation and infection in cell lines and primary cells. DDX42 overexpression inhibits HIV-1 infection, whereas expression of a dominant-negative mutant increases infection. Importantly, DDX42 also restricts LINE-1 retrotransposition and infection with other retroviruses and positive-strand RNA viruses, including CHIKV and SARS-CoV-2. However, DDX42 does not impact the replication of several negative-strand RNA viruses, arguing against an unspecific effect on target cells, which is confirmed by RNA-seq analysis. Proximity ligation assays show DDX42 in ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-05-28T01:07:51.833Z","creation":"2025-04-19T22:47:07.804Z"},"accession":"S-EPMC9638865","cross_references":{"pubmed":["36161446"],"doi":["10.15252/embr.202154061"]}}