biostudies-literatureUnknown10Chu WPolycystic ovary syndrome (PCOS) is a common and complex disorder impairing female fertility, yet its etiology remains elusive. It is reported that circadian rhythm disruption might play a crucial role in PCOS pathologic progression. Here, in this research, we investigated the effect of environmental long-term circadian rhythm dysfunction and clarified its pathogenic mechanism in the development of PCOS, which might provide the targeted clinical strategies to patients with PCOS. Female SD rats were used to construct a circadian rhythm misalignment model with constant darkness (12/12-h dark/dark cycle), and the control group was kept under normal circadian rhythm exposure (12/12-h light/dark cycle) for 8 weeks. We measured their reproductive, endocrinal, and metabolic profiles at different Frontiers in cell and developmental biology954186https://www.ebi.ac.uk/biostudies/studies/S-EPMC9639332biostudies-literatureLong-term environmental exposure of darkness induces hyperandrogenism in PCOS <i>via</i> melatonin receptor 1A and aromatase reduction.PMC9639332Chan WYLi SLu GGeng XChu WWang DChen ZJDu YZhai JfalseLong-term environmental exposure of darkness induces hyperandrogenism in PCOS <i>via</i> melatonin receptor 1A and aromatase reduction.Polycystic ovary syndrome (PCOS) is a common and complex disorder impairing female fertility, yet its etiology remains elusive. It is reported that circadian rhythm disruption might play a crucial role in PCOS pathologic progression. Here, in this research, we investigated the effect of environmental long-term circadian rhythm dysfunction and clarified its pathogenic mechanism in the development of PCOS, which might provide the targeted clinical strategies to patients with PCOS. Female SD rats were used to construct a circadian rhythm misalignment model with constant darkness (12/12-h dark/dark cycle), and the control group was kept under normal circadian rhythm exposure (12/12-h light/dark cycle) for 8 weeks. We measured their reproductive, endocrinal, and metabolic profiles at different 2022-01-01T00:00:00Z20222025-04-19T23:16:00.347Z2025-02-19T00:31:47.007ZS-EPMC96393323635350910.3389/fcell.2022.954186