{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Berger M"],"funding":["Dutch Research Council (NWO)"],"pagination":["6556"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9640692"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The bacterium Escherichia coli initiates replication once per cell cycle at a precise volume per origin and adds an on average constant volume between successive initiation events, independent of the initiation size. Yet, a molecular model that can explain these observations has been lacking. Experiments indicate that E. coli controls replication initiation via titration and activation of the initiator protein DnaA. Here, we study by mathematical modelling how these two mechanisms interact to generate robust replication-initiation cycles. We first show that a mechanism solely based on titration generates stable replication cycles at low growth rates, but inevitably causes premature reinitiation events at higher growth rates. In this regime, the DnaA activation switch becomes essential for "],"journal":["Nature communications"],"pubmed_title":["Robust replication initiation from coupled homeostatic mechanisms."],"pmcid":["PMC9640692"],"funding_grant_id":["024.003.019"],"pubmed_authors":["Berger M","Wolde PRT"],"additional_accession":[]},"is_claimable":false,"name":"Robust replication initiation from coupled homeostatic mechanisms.","description":"The bacterium Escherichia coli initiates replication once per cell cycle at a precise volume per origin and adds an on average constant volume between successive initiation events, independent of the initiation size. Yet, a molecular model that can explain these observations has been lacking. Experiments indicate that E. coli controls replication initiation via titration and activation of the initiator protein DnaA. Here, we study by mathematical modelling how these two mechanisms interact to generate robust replication-initiation cycles. We first show that a mechanism solely based on titration generates stable replication cycles at low growth rates, but inevitably causes premature reinitiation events at higher growth rates. In this regime, the DnaA activation switch becomes essential for ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-04T19:27:35.475Z","creation":"2025-04-04T19:27:35.475Z"},"accession":"S-EPMC9640692","cross_references":{"pubmed":["36344507"],"doi":["10.1038/s41467-022-33886-6"]}}