biostudies-literatureLi NNational Natural Science Foundation of China1024235https://www.ebi.ac.uk/biostudies/studies/S-EPMC9640740biostudies-literatureUnknown13The role of normal gut microbiota in asthma or ovalbumin (OVA)-induced asthma tolerance (OT) remains unclear. Here, we established mouse models of asthma and OT followed by 2 weeks of antibiotic treatment, to clear the gut microbiota. Antibiotic treatment was found to alleviate allergic asthma accompanied with a reduction of invariant natural killer (iNKT) cells. By RNA-seq analysis, we found that β-adrenergic receptor (ADRB) genes, including <i>Adrb1</i>, <i>Adrb2</i>, and <i>Adrb3</i>, were downregulated in asthmatic lungs, but these changes were reversed in OT lungs. Moreover, <i>Adrb2</i> and <i>Adrb3</i> were significantly upregulated in asthmatic lungs after antibiotic treatment. Surprisingly, blocking ADRB with propranolol relieved allergic asthma while reducing T helper 2 (Th2) andFrontiers in immunologyOral antibiotics relieve allergic asthma in post-weaning mice <i>via</i> reducing iNKT cells and function of ADRB2.PMC964074082070024, 91942305, 81970075He YJie ZChen JZhang MShi TSu XXie SLi NfalseOral antibiotics relieve allergic asthma in post-weaning mice <i>via</i> reducing iNKT cells and function of ADRB2.The role of normal gut microbiota in asthma or ovalbumin (OVA)-induced asthma tolerance (OT) remains unclear. Here, we established mouse models of asthma and OT followed by 2 weeks of antibiotic treatment, to clear the gut microbiota. Antibiotic treatment was found to alleviate allergic asthma accompanied with a reduction of invariant natural killer (iNKT) cells. By RNA-seq analysis, we found that β-adrenergic receptor (ADRB) genes, including <i>Adrb1</i>, <i>Adrb2</i>, and <i>Adrb3</i>, were downregulated in asthmatic lungs, but these changes were reversed in OT lungs. Moreover, <i>Adrb2</i> and <i>Adrb3</i> were significantly upregulated in asthmatic lungs after antibiotic treatment. Surprisingly, blocking ADRB with propranolol relieved allergic asthma while reducing T helper 2 (Th2) and2022-01-01T00:00:00Z20222025-05-18T13:37:25.287Z2025-05-18T13:37:25.287ZS-EPMC96407403638970610.3389/fimmu.2022.1024235