biostudies-literatureKanzler CRNICHD NIH HHSNational Institutes of HealthNational Institute of General Medical SciencesNIGMS NIH HHSNational Institute of Child Health and Human Development133-138https://www.ebi.ac.uk/biostudies/studies/S-EPMC9643680biostudies-literatureUnknown492BioID is a proximity labeling strategy whose goal is to identify in vivo protein-protein interactions. The central components of this strategy are modified biotin ligase enzymes that promiscuously add biotin groups to proteins in close proximity. The transferred biotin group provides a powerful tag for purification and thus identification of interacting proteins. While a variety of modified biotin ligases were created for BioID, the original enzymes were inefficient, required long incubation times, and high intracellular biotin concentrations for protein labeling. These limitations hinder the application of BioID in contexts such as developing embryos where processes such as cell division and cell fate decisions occur rapidly. Recently, a new biotin ligase called TurboID was developed thatDevelopmental biologyTurboID functions as an efficient biotin ligase for BioID applications in Xenopus embryos.PMC9643680R01HD091921T32GM135066T32 GM135066T32 GM008349R01 HD091921T32GM008349Kanzler CRSheets MDDowdle MEDonohue MfalseTurboID functions as an efficient biotin ligase for BioID applications in Xenopus embryos.BioID is a proximity labeling strategy whose goal is to identify in vivo protein-protein interactions. The central components of this strategy are modified biotin ligase enzymes that promiscuously add biotin groups to proteins in close proximity. The transferred biotin group provides a powerful tag for purification and thus identification of interacting proteins. While a variety of modified biotin ligases were created for BioID, the original enzymes were inefficient, required long incubation times, and high intracellular biotin concentrations for protein labeling. These limitations hinder the application of BioID in contexts such as developing embryos where processes such as cell division and cell fate decisions occur rapidly. Recently, a new biotin ligase called TurboID was developed that2022-01-01T00:00:00Z2022 Dec2025-04-26T09:40:15.361Z2025-04-06T13:08:54.062ZS-EPMC96436803627032710.1016/j.ydbio.2022.10.005