biostudies-literatureLeino ADAmerican Foundation for Pharmaceutical EducationNational Cancer InstituteNCI NIH HHS1141-1152https://www.ebi.ac.uk/biostudies/studies/S-EPMC9644239biostudies-literatureUnknown14(17)<b>Background:</b> Volumetric absorptive microsampling may reduce the blood collection burden associated with therapeutic drug monitoring of immunosuppression to prevent organ transplant rejection. This work describes the development of a laboratory and analytical technique for quantifying tacrolimus and mycophenolic acid (MPA) from the Tasso-M20™ in human whole blood using bead-based impact-assisted extraction. <b>Results:</b> The sampled blood volume was accurate with estimated volumes within <2% of the expected 20 μl. Recovery using impact-assisted extraction was 73-87% for MPA and 100% for tacrolimus and was hematocrit-independent for both analytes. The LC-MS/MS assay is precise and accurate within the acceptance criteria of 15%. <b>Conclusion:</b> The sampling and extraction procedureBioanalysisApplication of a new volumetric microsampling device for quantitative bioanalysis of immunosuppression.PMC9644239P30CA046592Pre-doctoral fellowshipPai MPTakyi-Williams JSun DWen BLeino ADfalseApplication of a new volumetric microsampling device for quantitative bioanalysis of immunosuppression.<b>Background:</b> Volumetric absorptive microsampling may reduce the blood collection burden associated with therapeutic drug monitoring of immunosuppression to prevent organ transplant rejection. This work describes the development of a laboratory and analytical technique for quantifying tacrolimus and mycophenolic acid (MPA) from the Tasso-M20™ in human whole blood using bead-based impact-assisted extraction. <b>Results:</b> The sampled blood volume was accurate with estimated volumes within <2% of the expected 20 μl. Recovery using impact-assisted extraction was 73-87% for MPA and 100% for tacrolimus and was hematocrit-independent for both analytes. The LC-MS/MS assay is precise and accurate within the acceptance criteria of 15%. <b>Conclusion:</b> The sampling and extraction procedure2022-01-01T00:00:00Z2022 Sep2025-05-18T12:59:05.324Z2025-05-18T12:59:05.324ZS-EPMC96442393620808210.4155/bio-2022-0155