biostudies-literatureThi Tran HVietnam Academy of Science and Technology389-398https://www.ebi.ac.uk/biostudies/studies/S-EPMC9645267biostudies-literatureUnknown50(5)Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes associated with <i>Dysosma difformis</i>-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. <i>Fusarium</i> was found the most prevalent genus with a colonization frequency of 11.11%, followed by <i>Trametes</i> (9.26%) and <i>Penicillium</i> (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specMycobiologyIsolation and Cytotoxic Potency of Endophytic Fungi Associated with <i>Dysosma difformis</i>, a Study for the Novel Resources of Podophyllotoxin.PMC9645267TDCN.01/20-22Thi Ninh NTien Do PHong Tran QHo Tran QThu Nguyen GThi Nguyen HHThi Tran HHoang Chu HBich Pham NfalseIsolation and Cytotoxic Potency of Endophytic Fungi Associated with <i>Dysosma difformis</i>, a Study for the Novel Resources of Podophyllotoxin.Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes associated with <i>Dysosma difformis</i>-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. <i>Fusarium</i> was found the most prevalent genus with a colonization frequency of 11.11%, followed by <i>Trametes</i> (9.26%) and <i>Penicillium</i> (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the spec2022-01-01T00:00:00Z20222026-05-28T00:47:52.365Z2024-11-21T01:37:48.883ZS-EPMC96452673640489610.1080/12298093.2022.2126166