biostudies-literatureUnknown13(1)Wang XCancer Research SocietyTerry Fox Research InstituteGenome British ColumbiaGenome CanadaGouvernement du Canada | Canadian Institutes of Health ResearchFollicular lymphoma (FL) is an indolent cancer of mature B-cells but with ongoing risk of transformation to more aggressive histology over time. Recurrent mutations associated with transformation have been identified; however, prognostic features that can be discerned at diagnosis could be clinically useful. We present here comprehensive profiling of both tumor and immune compartments in 155 diagnostic FL biopsies at single-cell resolution by mass cytometry. This revealed a diversity of phenotypes but included two recurrent patterns, one which closely resembles germinal center B-cells (GCB) and another which appears more related to memory B-cells (MB). GCB-type tumors are enriched for EZH2, TNFRSF14, and MEF2B mutations, while MB-type tumors contain increased follicular helper T-cells. MB-Nature communications6772https://www.ebi.ac.uk/biostudies/studies/S-EPMC9646774biostudies-literatureSingle-cell profiling reveals a memory B cell-like subtype of follicular lymphoma with increased transformation risk.PMC9646774May CShah SPIslam RKim JMorin RDSehn LHGracias DHilton LHirst MKusakabe MHung SScott DWAoki TNissen MWang XWeng APRoth AJSimkin GWong RFreeman CSavage KJChavez EACraig JWDuns GSegat GCTyshchenko KSteidl CJiang AKarsan ABrinkman RRSarkozy CfalseSingle-cell profiling reveals a memory B cell-like subtype of follicular lymphoma with increased transformation risk.Follicular lymphoma (FL) is an indolent cancer of mature B-cells but with ongoing risk of transformation to more aggressive histology over time. Recurrent mutations associated with transformation have been identified; however, prognostic features that can be discerned at diagnosis could be clinically useful. We present here comprehensive profiling of both tumor and immune compartments in 155 diagnostic FL biopsies at single-cell resolution by mass cytometry. This revealed a diversity of phenotypes but included two recurrent patterns, one which closely resembles germinal center B-cells (GCB) and another which appears more related to memory B-cells (MB). GCB-type tumors are enriched for EZH2, TNFRSF14, and MEF2B mutations, while MB-type tumors contain increased follicular helper T-cells. MB-2022-01-01T00:00:00Z2022 Nov2025-04-04T09:02:44.965Z2025-04-04T09:02:44.965ZS-EPMC96467743635192410.1038/s41467-022-34408-0