biostudies-literatureJiang XNational Natural Science Foundation of China1037607https://www.ebi.ac.uk/biostudies/studies/S-EPMC9646977biostudies-literatureUnknown12<i>Salmonella enterica</i> serovar Enteritidis is the most prevalent serotype that causes human infections worldwide. Consumption of <i>S.</i> Enteritidis-contaminated animal foods is a major source of human infections; however, eradicating bacteria from animals remains difficult. Therefore, it is necessary to develop new measures to prevent and control salmonellosis. Here, we used the outer-membrane vesicles (OMVs) of <i>S.</i> Enteritidis and assessed their protective efficacy and immune response in mice. Deletion of <i>tolR</i> in <i>S.</i> Enteritidis increased the production and size of OMVs compared to those in the wild type (WT) and Δ<i>rfaQ</i> strains. Intramuscular immunization with OMVs conferred greater protection than intraperitoneal and intranasal immunization. Moreover, OMVsFrontiers in cellular and infection microbiologyPreclinical evaluation of OMVs as potential vaccine candidates against <i>Salmonella enterica</i> serovar Enteritidis infection.PMC964697731920103015, 31730094, 32072821Chu CHong YJiang XGu JLi QWang ZJiao XfalsePreclinical evaluation of OMVs as potential vaccine candidates against <i>Salmonella enterica</i> serovar Enteritidis infection.<i>Salmonella enterica</i> serovar Enteritidis is the most prevalent serotype that causes human infections worldwide. Consumption of <i>S.</i> Enteritidis-contaminated animal foods is a major source of human infections; however, eradicating bacteria from animals remains difficult. Therefore, it is necessary to develop new measures to prevent and control salmonellosis. Here, we used the outer-membrane vesicles (OMVs) of <i>S.</i> Enteritidis and assessed their protective efficacy and immune response in mice. Deletion of <i>tolR</i> in <i>S.</i> Enteritidis increased the production and size of OMVs compared to those in the wild type (WT) and Δ<i>rfaQ</i> strains. Intramuscular immunization with OMVs conferred greater protection than intraperitoneal and intranasal immunization. Moreover, OMVs2022-01-01T00:00:00Z20222026-05-31T10:59:29.313Z2024-10-19T05:11:37.927ZS-EPMC96469773638916110.3389/fcimb.2022.1037607