{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(11)"],"submitter":["He A"],"pubmed_abstract":["Objective
Human papillomavirus (HPV) 6 and 11 are the two most common low-risk HPV subtypes, accounting for more than 90% of condyloma acuminatum. A simple, accurate and rapid screening method to be applied in community-level hospitals is in high demand.Methods
Endogenous internally controlled recombinase-assisted amplification (EIC-RAA) assays for HPV6 and 11 were performed in a single closed-tube at 39 °C within 30 min. The sensitivity and specificity of EIC-RAA were examined using recombinant plasmids and pre-tested HPV DNA. A total of 233 clinical samples were collected, and the DNA was extracted by traditional multi-step extraction, or sample releasing agent, before analysis by EIC-RAA. For comparison, HPV detection via Quantitative real-time PCR (qPCR) was also perfor"],"journal":["Heliyon"],"pagination":["e11323"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9647352"],"repository":["biostudies-literature"],"pubmed_title":["Development of field-applicable endogenous internally controlled recombinase-aided amplification (EIC-RAA) assays for the detection of human papillomavirus genotypes 6 and 11 using sample releasing agent."],"pmcid":["PMC9647352"],"pubmed_authors":["Li J","Tan H","Hu Y","Li F","Ma X","Fang C","Zhang M","Nie M","Zhang R","Ming Y","Rong X","He A","Shen X"],"additional_accession":[]},"is_claimable":false,"name":"Development of field-applicable endogenous internally controlled recombinase-aided amplification (EIC-RAA) assays for the detection of human papillomavirus genotypes 6 and 11 using sample releasing agent.","description":"Objective
Human papillomavirus (HPV) 6 and 11 are the two most common low-risk HPV subtypes, accounting for more than 90% of condyloma acuminatum. A simple, accurate and rapid screening method to be applied in community-level hospitals is in high demand.Methods
Endogenous internally controlled recombinase-assisted amplification (EIC-RAA) assays for HPV6 and 11 were performed in a single closed-tube at 39 °C within 30 min. The sensitivity and specificity of EIC-RAA were examined using recombinant plasmids and pre-tested HPV DNA. A total of 233 clinical samples were collected, and the DNA was extracted by traditional multi-step extraction, or sample releasing agent, before analysis by EIC-RAA. For comparison, HPV detection via Quantitative real-time PCR (qPCR) was also perfor","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2026-05-31T23:10:52.803Z","creation":"2024-10-17T17:11:01.768Z"},"accession":"S-EPMC9647352","cross_references":{"pubmed":["36387484"],"doi":["10.1016/j.heliyon.2022.e11323"]}}