{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guo Y"],"funding":["the Outstanding Young teacher sponsored by Qing Lan Project in Jiangsu Province","the National Natural Science Foundation of China"],"pagination":["1172-1178"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9648401"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(1)"],"pubmed_abstract":["No current RNA-targeted interference tools have been reported to simultaneously up and down-regulate different gene expressions. Here we characterized an RNA-targeted genetic regulatory strategy composed of a flap endonuclease 1 (FEN1) and specific mis-hairpin DNA probes (mis-hpDNA), to realize the orthogonal genetic regulation. By targeting mRNA, the strategy hindered the translation and silenced genes in human cells with efficiencies of ~60%. By targeting miRNA, the strategy prevented the combination of miRNA to its specific mRNA and increased this mRNA expression by about 3-folds. In combination, we simultaneously performed <i>CXCR4</i> gene knock-down (~50%) and <i>EGFR</i> gene activation (1.5-folds) in human cells. Although the functional property can be further improved, this RNA-ta"],"journal":["RNA biology"],"pubmed_title":["A novel strategy for orthogonal genetic regulation on different RNA targeted loci simultaneously."],"pmcid":["PMC9648401"],"funding_grant_id":["82150209","32071440"],"pubmed_authors":["Liu Y","Tian K","Wang P","Qi Z","Guo Y","Zhang X","Xu S","Qiang H","Zhou G","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"A novel strategy for orthogonal genetic regulation on different RNA targeted loci simultaneously.","description":"No current RNA-targeted interference tools have been reported to simultaneously up and down-regulate different gene expressions. Here we characterized an RNA-targeted genetic regulatory strategy composed of a flap endonuclease 1 (FEN1) and specific mis-hairpin DNA probes (mis-hpDNA), to realize the orthogonal genetic regulation. By targeting mRNA, the strategy hindered the translation and silenced genes in human cells with efficiencies of ~60%. By targeting miRNA, the strategy prevented the combination of miRNA to its specific mRNA and increased this mRNA expression by about 3-folds. In combination, we simultaneously performed <i>CXCR4</i> gene knock-down (~50%) and <i>EGFR</i> gene activation (1.5-folds) in human cells. Although the functional property can be further improved, this RNA-ta","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jan","modification":"2025-04-05T12:32:08.856Z","creation":"2025-04-05T12:32:08.856Z"},"accession":"S-EPMC9648401","cross_references":{"pubmed":["36350790"],"doi":["10.1080/15476286.2022.2141507"]}}