biostudies-literatureCortez NEHHS | NIH | National Cancer InstituteNCI NIH HHSUSDA | National Institute of Food and Agriculture951-965https://www.ebi.ac.uk/biostudies/studies/S-EPMC9648418biostudies-literatureUnknown2(9)Pancreatic ductal adenocarcinoma (PDAC) continues to be a major health problem. A ketogenic diet (KD), characterized by a very low carbohydrate and high fat composition, has gained attention for its anti-tumor potential. We evaluated the effect and mechanisms of feeding a strict KD alone or in combination with gemcitabine in the autochthonous LSL-Kras^G12D/+; LSL-Trp53 ^R172H/+; Pdx1-Cre (KPC) mouse model. For this purpose, both male and female pancreatic tumor-bearing KPC mice were allocated to a control diet (CD; %kcal: 70% carb, 14% protein, 16% fat), a KD (%kcal: 14% protein, 1% carb, 85% fat), a CD + gemcitabine (CG), or a KD + gemcitabine (KG) group. Mice fed a KD alone or in combination with gemcitabine showed significantly increased blood β-hydroxybutyrate levelCancer research communicationsA ketogenic diet in combination with gemcitabine increases survival in pancreatic cancer KPC mice.PMC9648418CA-D-NTR-2397-HP30CA093373P30 CA093373Muller MHwang CIPontifex MGWang FVauzour DRodriguez Lanzi CXu JMackenzie GGMatsukuma KHong BVChen SVahmani PCortez NERamsey JJfalseA ketogenic diet in combination with gemcitabine increases survival in pancreatic cancer KPC mice.Pancreatic ductal adenocarcinoma (PDAC) continues to be a major health problem. A ketogenic diet (KD), characterized by a very low carbohydrate and high fat composition, has gained attention for its anti-tumor potential. We evaluated the effect and mechanisms of feeding a strict KD alone or in combination with gemcitabine in the autochthonous LSL-Kras^G12D/+; LSL-Trp53 ^R172H/+; Pdx1-Cre (KPC) mouse model. For this purpose, both male and female pancreatic tumor-bearing KPC mice were allocated to a control diet (CD; %kcal: 70% carb, 14% protein, 16% fat), a KD (%kcal: 14% protein, 1% carb, 85% fat), a CD + gemcitabine (CG), or a KD + gemcitabine (KG) group. Mice fed a KD alone or in combination with gemcitabine showed significantly increased blood β-hydroxybutyrate level2022-01-01T00:00:00Z2022 Sep2026-05-10T06:13:12.021Z2025-04-19T12:10:45.177ZS-EPMC96484183638208610.1158/2767-9764.crc-22-0256