{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(1)"],"submitter":["Alam AA"],"pubmed_abstract":["<h4>Background</h4>Cholesterol oxidases (CHOs) have attracted enormous attention because of their wide biotechnological potential. The present study explores the production of CHOs by Streptomyces sp. AN. Evaluation of culture conditions affecting enzyme production, medium optimization and released metabolite characteristics were also investigated.<h4>Results</h4>The current work reports the isolation of 37 colonies (bacteria/actinobacteria) with different morphotypes from different soil/water samples. The isolate-coded AN was selected for its high potency for CHO production. Morphological characteristics and the obtained partial sequence of 16srRNA of AN showed 99.38% identity to Streptomyces sp. strain P12-37. Factors affecting CHO production were evaluated using Plackett-Burman (PB) and"],"journal":["Journal, genetic engineering & biotechnology"],"pagination":["156"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9649583"],"repository":["biostudies-literature"],"pubmed_title":["Production and statistical optimization of cholesterol-oxidase generated by Streptomyces sp. AN strain."],"pmcid":["PMC9649583"],"pubmed_authors":["Alam AA","Soliman NA","Abdel-Meguid DI","Sabry SA","Goda DA","El-Sharouny EE"],"additional_accession":[]},"is_claimable":false,"name":"Production and statistical optimization of cholesterol-oxidase generated by Streptomyces sp. AN strain.","description":"<h4>Background</h4>Cholesterol oxidases (CHOs) have attracted enormous attention because of their wide biotechnological potential. The present study explores the production of CHOs by Streptomyces sp. AN. Evaluation of culture conditions affecting enzyme production, medium optimization and released metabolite characteristics were also investigated.<h4>Results</h4>The current work reports the isolation of 37 colonies (bacteria/actinobacteria) with different morphotypes from different soil/water samples. The isolate-coded AN was selected for its high potency for CHO production. Morphological characteristics and the obtained partial sequence of 16srRNA of AN showed 99.38% identity to Streptomyces sp. strain P12-37. Factors affecting CHO production were evaluated using Plackett-Burman (PB) and","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-19T22:45:46.925Z","creation":"2025-04-19T22:45:46.925Z"},"accession":"S-EPMC9649583","cross_references":{"pubmed":["36355232"],"doi":["10.1186/s43141-022-00433-1"]}}