{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wamhoff EC"],"funding":["National Institute of Allergy and Infectious Diseases","Marble Center for Cancer Nanomedicine, Massachusetts Institute of Technology","Howard Hughes Medical Institute","Alexander von Humboldt-Stiftung","NIAID NIH HHS","Ragon Institute of MGH, MIT and Harvard","National Institute of Mental Health","National Institute of Biomedical Imaging and Bioengineering","Army Research Laboratory","Office of Naval Research"],"pagination":["8954-8966"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9649841"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(6)"],"pubmed_abstract":["Viruslike particles (VLPs) fabricated using wireframe DNA origami are emerging as promising vaccine and gene therapeutic delivery platforms due to their programmable nature that offers independent control over their size and shape, as well as their site-specific functionalization. As materials that biodegrade in the presence of endonucleases, specifically DNase I and II, their utility for the targeting of cells, tissues, and organs depends on their stability in vivo. Here, we explore minor groove binders (MGBs) as specific endonuclease inhibitors to control the degradation half-life of wireframe DNA origami. Bare, unprotected DNA-VLPs composed of two-helix edges were found to be stable in fetal bovine serum under typical cell culture conditions and in human serum for 24 h but degraded with"],"journal":["ACS nano"],"pubmed_title":["Controlling Nuclease Degradation of Wireframe DNA Origami with Minor Groove Binders."],"pmcid":["PMC9649841"],"funding_grant_id":["AI048240","R01 AI162307","R01-MH112694","UM1AI144462","UM1AI100663","N00014-21-1-4013","N00014-20-1-2084","N00014-16-1-2953","R21-EB026008","R01- AI162307","W911NF-18-2-0048"],"pubmed_authors":["Irvine DJ","Knappe GA","Kim HM","Farrell NP","Wamhoff EC","Huang H","Romanov A","Ginsburg E","Bathe M","Read BJ"],"additional_accession":[]},"is_claimable":false,"name":"Controlling Nuclease Degradation of Wireframe DNA Origami with Minor Groove Binders.","description":"Viruslike particles (VLPs) fabricated using wireframe DNA origami are emerging as promising vaccine and gene therapeutic delivery platforms due to their programmable nature that offers independent control over their size and shape, as well as their site-specific functionalization. As materials that biodegrade in the presence of endonucleases, specifically DNase I and II, their utility for the targeting of cells, tissues, and organs depends on their stability in vivo. Here, we explore minor groove binders (MGBs) as specific endonuclease inhibitors to control the degradation half-life of wireframe DNA origami. Bare, unprotected DNA-VLPs composed of two-helix edges were found to be stable in fetal bovine serum under typical cell culture conditions and in human serum for 24 h but degraded with","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Jun","modification":"2025-04-05T12:31:58.495Z","creation":"2025-04-05T12:31:58.495Z"},"accession":"S-EPMC9649841","cross_references":{"pubmed":["35640255"],"doi":["10.1021/acsnano.1c11575"]}}