biostudies-literatureXi HAustralia Research Council Future FellowshipNational Natural Science Foundation of ChinaAW Howard Memorial Trust Postgraduate Research FellowshipUniversity of Adelaide Research Training ScholarshipsDepartment of Industry, Science, Energy and Resourcesgigabyte38https://www.ebi.ac.uk/biostudies/studies/S-EPMC9650280biostudies-literatureUnknown2022<i>Vicia sativa</i> L. (common vetch, <i>n</i> = 6) is an annual, herbaceous, climbing legume, originating in the Fertile Crescent of the Middle East and now widespread in the Mediterranean basin, West, Central and Eastern Asia, North and South America. <i>V. sativa</i> is of economic importance as a forage legume in countries such as Australia, China, and the USA, and contributes valuable nitrogen to agricultural rotation cropping systems. To accelerate precision genome breeding and genomics-based selection of this legume, we present a chromosome-level reference genome sequence for <i>V. sativa</i>, constructed using a combination of long-read Oxford Nanopore sequencing, short-read Illumina sequencing, and high-throughput chromosome conformation data (CHiCAGO and Hi-C) analysis. The chromosome-level assembly of six pseudo-chromosomes has a total genome length of 1.65 Gbp, with a median contig length of 684 Kbp. BUSCO analysis of the assembly demonstrated very high completeness of 98% of the dicotyledonous orthologs. RNA-seq analysis and gene modelling enabled the annotation of 53,218 protein-coding genes. This <i>V. sativa</i> assembly will provide insights into vetch genome evolution and be a valuable resource for genomic breeding, genetic diversity and for understanding adaption to diverse arid environments.GigaByte (Hong Kong, China)Chromosome-level assembly of the common vetch <i>(Vicia sativa)</i> reference genome.PMC9650280ACSRF 4818731722055FT130100525Xi HNguyen VLiu ZSearle IRWard CfalseChromosome-level assembly of the common vetch <i>(Vicia sativa)</i> reference genome.<i>Vicia sativa</i> L. (common vetch, <i>n</i> = 6) is an annual, herbaceous, climbing legume, originating in the Fertile Crescent of the Middle East and now widespread in the Mediterranean basin, West, Central and Eastern Asia, North and South America. <i>V. sativa</i> is of economic importance as a forage legume in countries such as Australia, China, and the USA, and contributes valuable nitrogen to agricultural rotation cropping systems. To accelerate precision genome breeding and genomics-based selection of this legume, we present a chromosome-level reference genome sequence for <i>V. sativa</i>, constructed using a combination of long-read Oxford Nanopore sequencing, short-read Illumina sequencing, and high-throughput chromosome conformation data (CHiCAGO and Hi-C) analysis. The chromosome-level assembly of six pseudo-chromosomes has a total genome length of 1.65 Gbp, with a median contig length of 684 Kbp. BUSCO analysis of the assembly demonstrated very high completeness of 98% of the dicotyledonous orthologs. RNA-seq analysis and gene modelling enabled the annotation of 53,218 protein-coding genes. This <i>V. sativa</i> assembly will provide insights into vetch genome evolution and be a valuable resource for genomic breeding, genetic diversity and for understanding adaption to diverse arid environments.2022-01-01T00:00:00Z20222025-04-04T14:17:53.306Z2025-02-19T02:23:59.385ZS-EPMC96502803682452410.46471/gigabyte.38