biostudies-literatureUnknown2021Shirasawa KKazusa DNA Research Institute FoundationDepartment of Science and Technology of the Government of IndiaJapan Society for the Promotion of ScienceHorsegram (<i>Macrotyloma uniflorum</i> [Lam.] Verdc.) is an underutilized warm-season diploid legume (2<i>n</i> = 20, 22). Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of global climate change. In recognition of its potential as a crop species, we generated and analyzed a draft genome sequence for a horsegram variety, HPK-4. Ten chromosome-scale pseudomolecules were created by aligning Illumina scaffold sequences onto a linkage map. The total length of the ten pseudomolecules was 259.2 Mbp, covering 89% of the total length of the assembled sequences. A total of 36,105 genes were predicted on the assembled sequences. Diversity analysis of 89 horsegram accessions by dd-RAD-Seq identified 277 single nucleotide pGigaByte (Hong Kong, China)gigabyte30https://www.ebi.ac.uk/biostudies/studies/S-EPMC9650294biostudies-literatureA chromosome-scale draft genome sequence of horsegram (<i>Macrotyloma uniflorum</i>).PMC9650294Nagano SIsobe SSharma TChahota RHirakawa HNagasaki HShirasawa KfalseA chromosome-scale draft genome sequence of horsegram (<i>Macrotyloma uniflorum</i>).Horsegram (<i>Macrotyloma uniflorum</i> [Lam.] Verdc.) is an underutilized warm-season diploid legume (2<i>n</i> = 20, 22). Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of global climate change. In recognition of its potential as a crop species, we generated and analyzed a draft genome sequence for a horsegram variety, HPK-4. Ten chromosome-scale pseudomolecules were created by aligning Illumina scaffold sequences onto a linkage map. The total length of the ten pseudomolecules was 259.2 Mbp, covering 89% of the total length of the assembled sequences. A total of 36,105 genes were predicted on the assembled sequences. Diversity analysis of 89 horsegram accessions by dd-RAD-Seq identified 277 single nucleotide p2021-01-01T00:00:00Z20212025-04-04T14:17:10.414Z2025-02-19T02:23:57.481ZS-EPMC96502943682433310.46471/gigabyte.30