biostudies-literatureXiang HJiaxing Public Welfare Research ProjectNational Natural Science Foundation of ChinaZhejiang Provincial Natural Science Foundation7499https://www.ebi.ac.uk/biostudies/studies/S-EPMC9653583biostudies-literatureUnknown27(21)Hydrogen sulfide (H₂S) as small molecular signal messenger plays key functions in numerous biological processes. The imaging detection of intracellular hydrogen sulfide is of great significance. In this work, a ratiometric fluorescent probe <b>BH</b> based on an asymmetric BODIPY dye for detection of H₂S was designed and synthesized. After the interaction with hydrogen sulfide, probe display colorimetric and ratiometric fluorescence response, with its maximum emission fluorescence wavelength red-shifted from 542 nm to 594 nm, which is attributed to the sequential nucleophilic reaction of H₂S leading to enhanced molecular conjugation after ring formation of the BODIPY skeleton. A special response mechanism has been fully investigated by NMR titration and MS,Molecules (Basel, Switzerland)A New Ratiometric Fluorescent Probe Based on BODIPY for Highly Selective Detection of Hydrogen Sulfide.PMC9653583LY20B0600092021AY100702207108221606102Li YYe TXiang HLin YLiu DZhou HWang JLi LfalseA New Ratiometric Fluorescent Probe Based on BODIPY for Highly Selective Detection of Hydrogen Sulfide.Hydrogen sulfide (H₂S) as small molecular signal messenger plays key functions in numerous biological processes. The imaging detection of intracellular hydrogen sulfide is of great significance. In this work, a ratiometric fluorescent probe <b>BH</b> based on an asymmetric BODIPY dye for detection of H₂S was designed and synthesized. After the interaction with hydrogen sulfide, probe display colorimetric and ratiometric fluorescence response, with its maximum emission fluorescence wavelength red-shifted from 542 nm to 594 nm, which is attributed to the sequential nucleophilic reaction of H₂S leading to enhanced molecular conjugation after ring formation of the BODIPY skeleton. A special response mechanism has been fully investigated by NMR titration and MS,2022-01-01T00:00:00Z2022 Nov2025-04-25T23:23:23.731Z2025-04-06T09:22:32.159ZS-EPMC96535833636432510.3390/molecules27217499