{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sun Q"],"funding":["First Hospital of Jilin University","Project of Jilin Provincial Department of science and technology"],"pagination":["7619"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9655109"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(21)"],"pubmed_abstract":["A simple sample preprocessing method was developed for the quantitative determination of amantadine (AMT) in human plasma by liquid chromatography-tandem mass spectrometry cubed (LC-MS<sup>3</sup>). The LC-MS<sup>3</sup> system comprised a Shimadzu Exion LC-20AD HPLC pump coupled with a QTRAP 5500 mass spectrometer. First, the plasma samples were pretreated using acetonitrile as the extracting solution to precipitate protein. Next, amantadine and amantadine-d<sub>15</sub> (AMT-d<sub>15</sub>) were separated on an Agilent Poroshell 120 SB-C18 column (4.6 mm × 50 mm, 2.7 μm) using isocratic elution with solvent A (70% 0.1% formic acid) and solvent B (30% acetonitrile) at a flow rate of 0.8 mL/min. The total run time for each sample was 3 min. The system used triple-stage fragmentation transi"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Comparison of LC-MS<sup>3</sup> and LC-MRM Methods for Quantifying Amantadine and Its Application in Therapeutic Amantadine Monitoring in Human Plasma."],"pmcid":["PMC9655109"],"funding_grant_id":["20200404167YY","JDYY2021-A0009"],"pubmed_authors":["Liu Y","Li Y","Cao H","Sun Q","Huang J"],"additional_accession":[]},"is_claimable":false,"name":"Comparison of LC-MS<sup>3</sup> and LC-MRM Methods for Quantifying Amantadine and Its Application in Therapeutic Amantadine Monitoring in Human Plasma.","description":"A simple sample preprocessing method was developed for the quantitative determination of amantadine (AMT) in human plasma by liquid chromatography-tandem mass spectrometry cubed (LC-MS<sup>3</sup>). The LC-MS<sup>3</sup> system comprised a Shimadzu Exion LC-20AD HPLC pump coupled with a QTRAP 5500 mass spectrometer. First, the plasma samples were pretreated using acetonitrile as the extracting solution to precipitate protein. Next, amantadine and amantadine-d<sub>15</sub> (AMT-d<sub>15</sub>) were separated on an Agilent Poroshell 120 SB-C18 column (4.6 mm × 50 mm, 2.7 μm) using isocratic elution with solvent A (70% 0.1% formic acid) and solvent B (30% acetonitrile) at a flow rate of 0.8 mL/min. The total run time for each sample was 3 min. The system used triple-stage fragmentation transi","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-25T23:36:37.709Z","creation":"2025-04-06T09:23:49.302Z"},"accession":"S-EPMC9655109","cross_references":{"pubmed":["36364446"],"doi":["10.3390/molecules27217619"]}}