biostudies-literatureSun QFirst Hospital of Jilin UniversityProject of Jilin Provincial Department of science and technology7619https://www.ebi.ac.uk/biostudies/studies/S-EPMC9655109biostudies-literatureUnknown27(21)A simple sample preprocessing method was developed for the quantitative determination of amantadine (AMT) in human plasma by liquid chromatography-tandem mass spectrometry cubed (LC-MS³). The LC-MS³ system comprised a Shimadzu Exion LC-20AD HPLC pump coupled with a QTRAP 5500 mass spectrometer. First, the plasma samples were pretreated using acetonitrile as the extracting solution to precipitate protein. Next, amantadine and amantadine-d₁₅ (AMT-d₁₅) were separated on an Agilent Poroshell 120 SB-C18 column (4.6 mm × 50 mm, 2.7 μm) using isocratic elution with solvent A (70% 0.1% formic acid) and solvent B (30% acetonitrile) at a flow rate of 0.8 mL/min. The total run time for each sample was 3 min. The system used triple-stage fragmentation transiMolecules (Basel, Switzerland)Comparison of LC-MS³ and LC-MRM Methods for Quantifying Amantadine and Its Application in Therapeutic Amantadine Monitoring in Human Plasma.PMC965510920200404167YYJDYY2021-A0009Liu YLi YCao HSun QHuang JfalseComparison of LC-MS³ and LC-MRM Methods for Quantifying Amantadine and Its Application in Therapeutic Amantadine Monitoring in Human Plasma.A simple sample preprocessing method was developed for the quantitative determination of amantadine (AMT) in human plasma by liquid chromatography-tandem mass spectrometry cubed (LC-MS³). The LC-MS³ system comprised a Shimadzu Exion LC-20AD HPLC pump coupled with a QTRAP 5500 mass spectrometer. First, the plasma samples were pretreated using acetonitrile as the extracting solution to precipitate protein. Next, amantadine and amantadine-d₁₅ (AMT-d₁₅) were separated on an Agilent Poroshell 120 SB-C18 column (4.6 mm × 50 mm, 2.7 μm) using isocratic elution with solvent A (70% 0.1% formic acid) and solvent B (30% acetonitrile) at a flow rate of 0.8 mL/min. The total run time for each sample was 3 min. The system used triple-stage fragmentation transi2022-01-01T00:00:00Z2022 Nov2025-04-25T23:36:37.709Z2025-04-06T09:23:49.302ZS-EPMC96551093636444610.3390/molecules27217619